ABSTRACT The cultivar ‘G × N-9’ is an almond x peach hybrid rootstock, the main characteristic of which is resistance to several root-knot nematodes (Meloidogyne spp.). Although its propagation by hardwood cutting in the fall is recommended, optimization of ‘G × N-9’ in vitro propagation is currently needed. Thus, the objective of this study was to evaluate the effect of the cytokinins [6-benzylaminopurine (BAP) and Kinetin (KIN)], and the carbohydrate sources (sucrose and sorbitol) on shoot proliferation and elongation, as well as the influence of indole-3-butyric acid (IBA) on in vitro rooting and acclimatization of ‘G × N-9’ rootstock. This study reports enhanced on shoot proliferation and elongation based on the interaction between BAP and KIN. When comparing the effects of sorbitol and sucrose, sorbitol at 15 and 30 mg L−1 was the most effective for the induction of shoot proliferation (5.75 ± 0.19 and 6.39 ± 0.21 shoots per explant, respectively). The highest number of roots per explant was in QL medium supplemented with 0.6 and 1.2 mg L−1 IBA. After 30 days of acclimatization, the treated-plants with 1.2 mg L−1 IBA showed the smallest root length, but the higher number of roots per explant (8.35 ± 0.41). The successful acclimatization of plantlets depended on the use of IBA during in vitro culture, to induce a healthy and well-developed root system.

中文翻译：

---

细胞分裂素、碳水化合物源和生长素对'G×N-9'桃砧木体外繁殖的影响

摘要 栽培品种'G × N-9' 是杏仁 x 桃杂交砧木，其主要特征是对几种根结线虫（Meloidogyne spp.）的抗性。尽管建议在秋季通过硬木切割繁殖，但目前需要优化“G×N-9”体外繁殖。因此，本研究的目的是评估细胞分裂素 [6-苄氨基嘌呤 (BAP) 和激动素 (KIN)] 和碳水化合物来源（蔗糖和山梨糖醇）对芽增殖和伸长的影响，以及indole-3-butyric acid (IBA) 对 'G × N-9' 砧木体外生根和驯化的影响。该研究报告了基于 BAP 和 KIN 之间相互作用的芽增殖和伸长增强。在比较山梨糖醇和蔗糖的效果时，15 和 30 mg L-1 的山梨糖醇对诱导枝条增殖最有效（每个外植体分别为 5.75 ± 0.19 和 6.39 ± 0.21 枝）。每个外植体的最高根数是在补充有 0.6 和 1.2 mg L-1 IBA 的 QL 培养基中。驯化 30 天后，含有 1.2 mg L-1 IBA 的处理植物显示出最小的根长，但每个外植体的根数更高 (8.35 ± 0.41)。小苗的成功驯化取决于在体外培养过程中使用 IBA 来诱导健康和发育良好的根系。2 mg L-1 IBA 显示最小的根长度，但每个外植体的根数更高 (8.35 ± 0.41)。小苗的成功驯化取决于在体外培养过程中使用 IBA 来诱导健康和发育良好的根系。2 mg L-1 IBA 显示最小的根长度，但每个外植体的根数更高 (8.35 ± 0.41)。小苗的成功驯化取决于在体外培养过程中使用 IBA 来诱导健康和发育良好的根系。