ABSTRACT

The successful implantation depends on the favorable biocompatibility of the implant. In this paper,microgrooves on the surface of Ti-6Al-4V titanium alloy implants were made by laser processing technology,scanning electron microscopy and surface profilometer were used to observe the laser textured titanium samples,firstly. Then, the liquid-solid contact angle-measuring instrument was used to characterize the wettability of titanium samples. Finally,using a microplate reader and laser confocal microscope, the cytological behavior of cell proliferation, adhesion, differentiation, and cell morphology on the surface of different samples were studied.It showed that laser microgrooved surfaces had reduced liquid-solid contact angles,increased surface roughness,and better proliferation and differentiation of osteoblasts, while most cells grew along the microgrooves. These results indicate that the increased wettability provides a better growth environment for cell growth. Microgrooves are crucial in cell contact guidance, while their parameters control cell behavior and deserve an in-depth study.

摘要

成功的植入取决于植入物良好的生物相容性。本文采用激光加工技术在Ti-6Al-4V钛合金种植体表面制作微槽,首先利用扫描电镜和表面轮廓仪对激光织构钛样品进行观察。然后，利用液固接触角测量仪对钛样品的润湿性进行表征。最后,利用酶标仪和激光共聚焦显微镜,研究了不同样品表面细胞增殖、粘附、分化和细胞形态的细胞学行为。粗糙度，成骨细胞更好的增殖和分化，而大多数细胞沿着微槽生长。这些结果表明增加的润湿性为细胞生长提供了更好的生长环境。微槽在细胞接触引导中至关重要，而它们的参数控制着细胞行为，值得深入研究。