ABSTRACT

Dongxiang common wild rice (CWR) is extremely cold tolerant, which can grow at the northernmost latitudes. To take full advantage of Dongxiang CWR’s cold tolerance for rice production and breeding, it is important to obtain a deep understanding of the mechanism underlying its cold tolerance. In this study, the rhizospheric microbiome of CWR was investigated and compared with that of Leersia hexandra Swartz (LHS) in Dongxiang to clarify the role of the rhizospheric microbiome in CWR cold tolerance. The results showed that, compared with the LHS rhizospheric microbiome, the CWR rhizospheric microbiome was less diverse and smaller, as indicated by its lower diversity and richness indices and fewer detected groups, core genera, and indicator genera. However, a larger proportion of the CWR indicator genera and unique genera, including Acidiphilium, Mortierella, Glomus, etc. showed the ability to adapt to low temperatures, indicating that CWR was more likely to be associated with microbial groups that may enhance cold tolerance. The results indicated that the extreme cold tolerance of Dongxiang CWR benefited from its rhizospheric microbiome, and this cold tolerance was speculated to benefit more from key microbial groups (indicator genera and unique genera) than from microbial diversity.

摘要

东乡普通野生稻（CWR）具有极强的耐寒性，可以在最北纬地区生长。为充分利用东乡CWR的耐寒性进行水稻生产和育种，深入了解其耐寒性机制至关重要。在这项研究中，研究了 CWR 的根际微生物组，并与Leersia hexandra的根际微生物组进行了比较。东乡 Swartz (LHS) 阐明根际微生物组在 CWR 耐寒性中的作用。结果表明，与LHS根际微生物组相比，CWR根际微生物组的多样性和规模较小，多样性和丰富度指数较低，检测到的组、核心属和指示属较少。然而，更大比例的 CWR 指示属和独特属，包括嗜酸菌属、被孢霉属、Glomus等显示出适应低温的能力，表明CWR更可能与可能增强耐寒性的微生物群有关。结果表明，东乡西红花的极端耐寒性得益于其根际微生物组，推测这种耐寒性更多地得益于关键微生物群（指示属和独特属），而不是微生物多样性。