Polymer grafting from a metallo‐centered enzyme improves activity in non‐native environments,Polymer International

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Polymer grafting from a metallo‐centered enzyme improves activity in non‐native environments
Polymer International ( IF 2.9 ) Pub Date : 2020-09-21 , DOI: 10.1002/pi.6127
Marina Kovaliov _{1,

2} , Borui Zhang _{1,

2} , Dominik Konkolewicz ₃ , Katarzyna Szcześniak ₄ , Stefa Jurga ₅ , Saadyah Averick _{1,

2}

Affiliation

Metallo‐centered enzymes have found applications in environmental remediation, green synthesis and sensors. These enzymes destabilize at extreme pH, elevated temperature or high‐organic‐content conditions. For these enzymes to find more widespread adoption and use, stabilization in non‐native conditions is critical. We report the impact of grafting polymers from the surface of laccase, a metalloenzyme with a Cu active site, modified with a polymerization‐initiating species ligated to the protein's lysine residues. Initiators for continuous activator regeneration atom transfer radical polymerization was used to graft a small library of polymers from the protein. The impact of polymer grafting on enzyme activity as a function of pH, temperature or organic solvent was determined. While polymer grafting from laccases was found to increase enzymatic activity, we found that grafting high‐molecular‐weight dimethylaminoethyl methacrylate displayed the greatest enhancement to catalytic activity. The results demonstrate that polymer grafting can improve laccase activity in native and non‐native conditions. © 2020 Society of Industrial Chemistry

中文翻译：

以金属为中心的酶接枝聚合物可改善非天然环境中的活性

以金属为中心的酶已发现可用于环境修复，绿色合成和传感器。这些酶在极端pH，高温或高有机物含量的条件下会不稳定。为了使这些酶得到更广泛的采用和使用，在非天然条件下稳定至关重要。我们报道了从漆酶的表面接枝聚合物的影响，漆酶是一种具有Cu活性位点的金属酶，用连接到蛋白质赖氨酸残基的聚合引发物种修饰。用于连续活化剂再生的引发剂原子转移自由基聚合用于从蛋白质中接枝小的聚合物文库。确定了聚合物接枝对酶活性的影响，该酶活性是pH，温度或有机溶剂的函数。虽然发现了从漆酶接枝的聚合物可以增加酶的活性，但我们发现接枝高分子量的甲基丙烯酸二甲氨基乙酯显示出最大的催化活性增强。结果表明，聚合物接枝可以提高天然和非天然条件下的漆酶活性。©2020工业化学学会

更新日期：2020-09-21

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