Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01,Microbial Biotechnology

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Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-09-20 , DOI: 10.1111/1751-7915.13659
Hengxu Wang _{1,

2,

3} , Zhigang Wang _{1,

2,

3} , Zeping Liu _{1,

3} , Kexin Wang _{1,

3} , Weihui Xu _{1,

2,

3}

Affiliation

Myriocin, which is produced by Bacillus amyloliquefaciens LZN01, can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon). In the present study, the antifungal mechanism of myriocin against Fon was investigated with a focus on the effects of myriocin on the cell membrane. Myriocin decreased the membrane fluidity and destroyed the membrane integrity of Fon. Significant microscopic morphological changes, including conidial shrinkage, the appearance of larger vacuoles and inhomogeneity of electron density, were observed in myriocin‐treated cells. A membrane‐targeted mechanism of action was also supported by transcriptomic and proteomic analyses; a total of 560 common differentially expressed genes (DEGs) and 285 common differentially expressed proteins (DEPs) were identified. The DEGs were further verified by using RT‐qPCR. The combined analysis between the transcriptome and proteome revealed that the expression of some membrane‐related genes and proteins, mainly those related to sphingolipid metabolism, glycerophospholipid metabolism, steroid biosynthesis, ABC transporters and protein processing in the endoplasmic reticulum, was disordered. Myriocin affected the serine palmitoyl transferase (SPT) activity as evidenced through molecular docking. Our results indicate that myriocin has significant antifungal activity owing to its ability to induce membrane damage in Fon.

中文翻译：

Fusarium oxysporum 的膜破坏。 sp。解淀粉芽孢杆菌 LZN01 多球壳菌素诱导的尼维菌素

解淀粉芽孢杆菌LZN01 产生的多球菌素 (Myriocin) 可以抑制尖孢镰刀菌 (Fusarium oxysporum f) 的生长。 sp。尼维姆(Fon)。在本研究中，研究了多壳菌素针对 Fon 的抗真菌机制，重点关注多球菌素对细胞膜的影响。多球菌素降低了膜的流动性并破坏了 Fon 的膜完整性。在经多球壳菌素处理的细胞中观察到显着的微观形态变化，包括分生孢子收缩、出现较大的液泡和电子密度不均匀。转录组和蛋白质组分析也支持膜靶向作用机制；共鉴定出560个常见差异表达基因（DEG）和285个常见差异表达蛋白（DEP）。使用 RT-qPCR 进一步验证 DEG。转录组和蛋白质组联合分析发现，一些膜相关基因和蛋白表达紊乱，主要是与鞘脂代谢、甘油磷脂代谢、类固醇生物合成、ABC转运蛋白和内质网蛋白加工相关的基因和蛋白。通过分子对接证明，多球菌素影响丝氨酸棕榈酰转移酶 (SPT) 活性。我们的结果表明，多球菌素具有显着的抗真菌活性，因为它能够诱导 Fon 膜损伤。

更新日期：2020-09-20

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