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Poly‐ε‐Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2020-09-21 , DOI: 10.1002/admi.202001232 Rebecca Lace 1 , Kyle G. Doherty 1 , Debarun Dutta 2 , Mark D. P. Willcox 2 , Rachel L. Williams 1
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2020-09-21 , DOI: 10.1002/admi.202001232 Rebecca Lace 1 , Kyle G. Doherty 1 , Debarun Dutta 2 , Mark D. P. Willcox 2 , Rachel L. Williams 1
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Microbial keratitis (MK) is a serious issue in many countries and is often caused by contact lens wear. Antimicrobial peptides (AMPs) are a potentially useful tool for creating antimicrobial surfaces in light of increasing antibiotic resistance. Poly‐ε‐lysine (pεK) is an AMP that has been used extensively as a food preservative and Mel4 has recently been synthesized and studied as an antimicrobial coating for contact lenses. A hydrogel synthesized of pεK cross‐linked with biscarboxylic acids provides a potential lens material which has many surface free amines, that can be subsequently used to attach additional AMPs, creating an antimicrobial lens. The aim of this study is to investigate pεK hydrogels against a clinical strain of Pseudomonas aeruginosa (P. aeruginosa) for preventing or treating MK. Covalent attachment of AMPs is investigated and confirmed by fluorescently tagged peptides. Bound pεK effectively reduces the number of adherent P. aeruginosa in vitro (>3 log). In ex vivo studies positive antimicrobial activity is observed on bare pεK hydrogels and those with additionally bound pεK or Mel4; lenses allow the maintenance of the corneal epithelium. A pεK hydrogel contact lens with additional AMPs can be a therapeutic tool to reduce the incidence of MK.
中文翻译：
新型肽水凝胶绷带隐形眼镜上的聚ε-赖氨酸或Mel4抗菌表面修饰
微生物角膜炎（MK）在许多国家是一个严重的问题，通常是由隐形眼镜的佩戴引起的。鉴于抗菌素耐药性的增加，抗菌肽（AMP）是创建抗菌表面的潜在有用工具。聚ε-赖氨酸（pεK）是一种AMP，已广泛用作食品防腐剂，最近合成并研究了Mel4作为隐形眼镜的抗菌涂层。由pεK与双羧酸交联合成的水凝胶提供了一种潜在的镜片材料，该材料具有许多表面自由胺，可随后用于连接其他AMP，从而形成抗菌镜片。这项研究的目的是研究针对临床铜绿假单胞菌（P. aeruginosa）菌株的pεK水凝胶用于预防或治疗MK。对AMPs的共价连接进行了研究，并通过荧光标记的肽进行了确认。结合的pεK可有效减少体外附着的铜绿假单胞菌的数量（> 3 log）。在离体研究中，在裸露的pεK水凝胶和另外结合了pεK或Mel4的凝胶上观察到了积极的抗菌活性；晶状体可以维持角膜上皮。具有附加AMP的pεK水凝胶隐形眼镜可以作为降低MK发生率的治疗工具。
更新日期：2020-11-06
中文翻译：
新型肽水凝胶绷带隐形眼镜上的聚ε-赖氨酸或Mel4抗菌表面修饰
微生物角膜炎（MK）在许多国家是一个严重的问题，通常是由隐形眼镜的佩戴引起的。鉴于抗菌素耐药性的增加，抗菌肽（AMP）是创建抗菌表面的潜在有用工具。聚ε-赖氨酸（pεK）是一种AMP，已广泛用作食品防腐剂，最近合成并研究了Mel4作为隐形眼镜的抗菌涂层。由pεK与双羧酸交联合成的水凝胶提供了一种潜在的镜片材料，该材料具有许多表面自由胺，可随后用于连接其他AMP，从而形成抗菌镜片。这项研究的目的是研究针对临床铜绿假单胞菌（P. aeruginosa）菌株的pεK水凝胶用于预防或治疗MK。对AMPs的共价连接进行了研究，并通过荧光标记的肽进行了确认。结合的pεK可有效减少体外附着的铜绿假单胞菌的数量（> 3 log）。在离体研究中，在裸露的pεK水凝胶和另外结合了pεK或Mel4的凝胶上观察到了积极的抗菌活性；晶状体可以维持角膜上皮。具有附加AMP的pεK水凝胶隐形眼镜可以作为降低MK发生率的治疗工具。
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