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A Spheroid‐Forming Hybrid Gold Nanostructure Platform That Electrochemically Detects Anticancer Effects of Curcumin in a Multicellular Brain Cancer Model
Small ( IF 13.0 ) Pub Date : 2020-09-20 , DOI: 10.1002/smll.202002436
Intan Rosalina Suhito 1 , Novi Angeline 1 , Kwang-Ho Lee 1 , Huijung Kim 1 , Chun Gwon Park 2 , Zhengtang Luo 3 , Tae-Hyung Kim 1, 4
Affiliation  

In this study, a multifunctional platform that enables the highly efficient formation of 3D multicellular cancer spheroids and precise real‐time assessments of the anticancer effects of curcumin in a brain tumor coculture model is reported. A highly conductive gold nanostructure (HCGN) is fabricated to facilitate cancer spheroid formation without using anti‐cell adhesion molecules. A neuroblastoma (SH‐SY5Y) and glioblastoma (U‐87MG) coculture model is generated on HCGN with a specific cell‐to‐cell ratio (SH‐SY5Y: U‐87MG = 1:1), and their redox behaviors are successfully measured without destroying the distinct 3D structure of the multicellular spheroids. Using electrochemical signals as an indicator of spheroid viability, the effects of potential anticancer compounds on cocultured spheroids are further assessed. Remarkably, decreased cell viability in 3D spheroids caused by a low concentration of curcumin (30 µM) is detectable using the electrochemical method (29.4%) but not with a conventional colorimetric assay (CCK‐8). The detection is repeated more than ten times for both short‐ (63 h) and long‐term cultivation (144 h) without damaging the spheroids, enabling real‐time, non‐destructive pharmacokinetic analysis of various drug candidates. Therefore, it can be concluded that the hybrid platform is a highly promising, precise, and high‐throughput drug screening tool based on 3D cell cultivation.

中文翻译:

形成球体的混合金纳米结构平台,可电化学检测姜黄素在多细胞脑癌模型中的抗癌作用。

在这项研究中,报告了一个多功能平台,该平台能够高效形成3D多细胞癌症球体,并在脑肿瘤共培养模型中精确实时评估姜黄素的抗癌作用。高导电金纳米结构(HCGN)可以在不使用抗细胞粘附分子的情况下促进癌症球体的形成。在HCGN上以特定的细胞对细胞比例(SH-SY5Y:U-87MG = 1:1)生成了神经母细胞瘤(SH-SY5Y)和胶质母细胞瘤(U-87MG)共培养模型,并成功测量了它们的氧化还原行为而不会破坏多细胞球体的独特3D结构。使用电化学信号作为球体生存能力的指标,进一步评估了潜在的抗癌化合物对共培养球体的影响。值得注意的是 低浓度的姜黄素(30 µM)导致3D球体细胞活力降低,可以使用电化学方法(29.4%)进行检测,而常规比色法(CCK-8)则无法检测到。短时(63 h)和长期培养(144 h)重复检测十次以上,而不会损坏球体,从而可以对各种候选药物进行实时,非破坏性的药代动力学分析。因此,可以得出结论,混合平台是基于3D细胞培养的高度有前途,精确且高通量的药物筛选工具。短时(63 h)和长期培养(144 h)重复检测十次以上,而不会损坏球体,从而可以对各种候选药物进行实时,非破坏性的药代动力学分析。因此,可以得出结论,混合平台是基于3D细胞培养的高度有前途,精确且高通量的药物筛选工具。短时(63 h)和长期培养(144 h)重复检测十次以上,而不会损坏球体,从而可以对各种候选药物进行实时,非破坏性的药代动力学分析。因此,可以得出结论,混合平台是基于3D细胞培养的高度有前途,精确且高通量的药物筛选工具。
更新日期:2020-09-20
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