On‐Chip Rotation of Caenorhabditis elegans Using Microfluidic Vortices,Advanced Materials Technologies

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On‐Chip Rotation of Caenorhabditis elegans Using Microfluidic Vortices
Advanced Materials Technologies ( IF 6.4 ) Pub Date : 2020-09-21 , DOI: 10.1002/admt.202000575
Peng Pan _{1,

2} , John D. Laver ₃ , Zhen Qin ₁ , Yuxiao Zhou ₁ , Ran Peng ₁ , Lijun Zhao ₄ , Hui Xie ₄ , John A. Calarco ₃ , Xinyu Liu _{1,

5}

Affiliation

Precise and controllable rotation of small biological samples is essential to many biological and medical applications. This paper reports, an easy‐to‐use microfluidic device to rotate single nematode worm Caenorhabditis elegans in a reliable and controllable fashion, which was enabled by on‐chip generation of stable microscale vortices inside a worm‐loaded microchannel by fluidic shear stress. To test the capability of the proposed device, single worms were successfully rotated in continuous and stepwise modes. Using this device, clear visualization of all dopaminergic neurons in the head of a C. elegans was demonstrated by capturing fluorescence images of the worm body at several rotational angles. Multiple perspectives of individual neurons of a multi‐color fluorescent transgenic worm were also obtained at high resolution using laser‐scanning confocal microscopy. The results show that this microfluidic rotation device provides a simple solution to overcoming limitations of confocal microscopy when imaging relatively thick tissue samples such as an adult C. elegans, and is compatible with multiple fluorescent proteins with different spectral properties. With its controllability, precision, and simplicity in fabrication and operation, this microfluidic device has important utility in model organism studies.

中文翻译：

秀丽隐杆线虫的芯片上旋转使用微流涡旋。

小生物样品的精确且可控的旋转对于许多生物和医学应用至关重要。本文报道了一种易于使用的微流控设备，以可靠且可控的方式旋转单个线虫蠕虫秀丽隐杆线虫，这是通过在蠕动加载的微通道内通过流体剪切应力在芯片上生成稳定的微尺度涡旋而实现的。为了测试所提出设备的功能，成功地以连续和逐步模式旋转了单个蠕虫。使用此设备，可清晰显示秀丽隐杆线虫头部中的所有多巴胺能神经元通过在多个旋转角度捕获蠕虫体的荧光图像证明了这一点。还使用激光扫描共聚焦显微镜以高分辨率获得了多色荧光转基因蠕虫单个神经元的多种视角。结果表明，当对较厚的组织样本（例如成年秀丽隐杆线虫）成像时，这种微流体旋转装置为克服共聚焦显微镜的局限性提供了简单的解决方案，并且与具有不同光谱特性的多种荧光蛋白兼容。由于其可控制性，精确性以及制造和操作的简便性，这种微流体装置在模型生物研究中具有重要的用途。

更新日期：2020-09-21

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