AIM Previous studies have shown that 5- hydroxytryptophan (5-HTP) and exercise play an important role in the synthesis of serotonin independently. The effects of the treadmill exercise and 5- hydroxytryptophan (5-HTP) on seizure mechanisms created by epileptiform activity with penicillin model were investigated in rats. METHOD A total of 28 male albino Wistar rats were randomly divided into four groups: exercise (Ex), Control (Cnt), 5-hydroxytryptophan (5htp) and 5-hydroxytryptophan + exercise (5htpEx) groups. Treadmill exercise and gavage (25 mg/kg/day) were administered five days a week for ten weeks. Electrocorticogram data were recorded for 3 h at the end of the protocol using the Power-Lab data acquisition system. Spike frequency, amplitude, and latency time were analyzed offline. The significant differences among the groups were evaluated by one-way analysis of variance (ANOVA). RESULTS Spike frequency was observed at the highest level from the 20th minute in the Cnt group, and this continued until the end of the recording. The 5-HTP alone group did not affect epileptiform activity. At the 80th minute of penicillin injection, the epileptiform activity in the 5htpEx group decreased significantly compared with the Cnt, and this significance continued until the 110th minute. There was no statistical difference in the amplitude values of the groups. The 5htpEx group was significantly higher than both the Cnt and Ex group in the seizure latency times. CONCLUSIONS It was determined that exercise reduced the spike number and delayed seizure significantly by potentiating the effect of 5-HTP. Given that 5-HTP used in combination with exercise can perform useful actions such as reducing seizure sensitivity and consequently improving the quality of life for individuals with epilepsy, it may be a potential candidate for the treatment of epilepsy in nonpharmacological methods.

中文翻译：

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体育锻炼和 5-羟色氨酸（血清素合成的前体）可降低青霉素诱发的癫痫样活动

目的 以前的研究表明，5-羟色氨酸 (5-HTP) 和运动独立地在血清素的合成中起重要作用。在大鼠中研究了跑步机运动和 5-羟色氨酸 (5-HTP) 对青霉素模型癫痫样活动产生的癫痫发作机制的影响。方法 28只雄性白化Wistar大鼠随机分为4组：运动组（Ex）、对照组（Cnt）、5-羟色氨酸（5htp）组和5-羟色氨酸+运动组（5htpEx）。每周 5 天进行跑步机运动和灌胃（25 毫克/千克/天），持续 10 周。使用 Power-Lab 数据采集系统在协议结束时记录皮层电图数据 3 小时。离线分析尖峰频率、幅度和潜伏时间。通过单因素方差分析（ANOVA）评估各组之间的显着差异。结果 Cnt 组从第 20 分钟开始观察到最高水平的尖峰频率，并一直持续到记录结束。5-HTP 单独组不影响癫痫样活动。在青霉素注射第80分钟，5htpEx组癫痫样活性较Cnt显着下降，这种显着性一直持续到第110分钟。各组振幅值无统计学差异。5htpEx 组的癫痫潜伏期显着高于 Cnt 和 Ex 组。结论 确定运动通过增强 5-HTP 的作用显着减少了峰值数量和延迟癫痫发作。