Implanted tissue engineering devices interact with the host tissue through their surface in the first instance. Surface chemistry triggers cell activities that stimulate bone tissue-formation mechanisms for osteoblast maturation. In this work, the bioactivity of binary Ti40Nb and Ti10Sn and ternary Ti-10Nb-5Sn alloys, candidates for bioengineering applications, has been studied on their surface with a view to establish their osteogenic potential compared to that of c.p. Ti. Cellular population growth was used to assess proliferative and differentiative phenotypes (via protein and Alkaline Phosphatase markers), coupled with gene expression (i.e. Runx2 and OCN) to confirm maturation. The results show that Sn-containing alloys support cell bioactivity, increase metabolic activity (i.e. metabolites content) that indicate their preferred glycolytic pathway, promote cell attachment, differentiation and osteoblast maturation. Ti40Nb, although also non-cytotoxic, retards osteoblastic differentiation and maturation. To elucidate the features that underpin this difference, their physical (i.e. wettability, electrical state near the surface) and chemical properties (i.e. oxide layer thickness and composition) were analysed independently from topology and roughness. It was concluded that composition (esp. TiO₂% content) is a more important factor than wettability and oxide layer thickness, and that although a negatively-charged surface (represented by the surface ζ potential) was preferential for cell bioactivity given its protein-adsorption readiness, its magnitude was not a defining cause.

首先，植入的组织工程设备通过其表面与宿主组织相互作用。表面化学触发细胞活动，刺激成骨细胞成熟的骨组织形成机制。在这项工作中，二元Ti 40Nb和Ti的生物活性已经对生物工程应用的候选材料10Sn和三元Ti-10Nb-5Sn合金进行了研究，以建立与cp Ti相比的成骨潜能。细胞群体的生长用于评估增殖和分化表型（通过蛋白质和碱性磷酸酶标记），并与基因表达（即Runx2和OCN）结合以确认成熟。结果表明，含锡合金支持细胞生物活性，增加表明其优选糖酵解途径的代谢活性（即代谢物含量），促进细胞附着，分化和成骨细胞成熟。钛40Nb虽然也没有细胞毒性，但会延迟成骨细胞的分化和成熟。为了阐明造成这种差异的特征，独立于拓扑结构和粗糙度分析了它们的物理性质（即，润湿性，表面附近的电态）和化学性质（即，氧化物层的厚度和组成）。结论是，组成（尤其是TiO ₂％的含量）是比润湿性和氧化物层厚度更重要的因素，尽管带负电荷的表面（由表面ζ电位表示）对细胞的生物活性具有优先作用，因为它具有蛋白质-吸附准备就绪，其大小不是决定性的原因。