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Real-time tracking the mitochondrial membrane potential by a mitochondria-lysosomes migration fluorescent probe with NIR-emissive AIE characteristics
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2020-09-21 , DOI: 10.1016/j.snb.2020.128929
Li Fan , Jinyin Ge , Qi Zan , Xiaodong Wang , Shuohang Wang , Yuewei Zhang , Wenjuan Dong , Shaomin Shuang , Chuan Dong

Mitochondrial membrane potential (MMP) is a critical parameter representing mitochondrial function and cellular activities. The development of a robust and convenient method for real-time tracking the MMP changes is still highly desirable and urgently needed. Herein, we presented a novel mitochondria-lysosomes migration fluorescent probe (CSP) with aggregation-induced emission (AIE) characteristics and near-infrared (NIR) solid-state emission for in situ real-time tracking MMP variations. The cationic probe CSP exclusively stained mitochondria with normal MMP; once MMP decreases, CSP could be released from mitochondria and migrated to lysosomes; with the recovery of MMP, CSP could move back to the mitochondria. Interestingly, the average colocalization coefficient (CLC) between CSP and a commercial lysosome-specific dye (LB-NIR) have been successfully served as a reliable and practical MMP parameter for real-time tracking the dynamic changes of MMP. Moreover, other unique features of AIEgens such as large Stokes shift (197 nm), high fluorescence quantum yield (9.8 %), excellent photostability and wash-free bioimaging, together make CSP a promising tool for real-time tracking MMP during the mitochondria-involved pathophysiologic processes.



中文翻译:

具有NIR发射AIE特性的线粒体-溶酶体迁移荧光探针实时跟踪线粒体膜电位

线粒体膜电位(MMP)是代表线粒体功能和细胞活性的关键参数。仍然非常需要并且迫切需要开发一种鲁棒且方便的方法来实时跟踪MMP变化。在此,我们提出了一个新颖的线粒体溶酶体移民工作荧光探针(CSP)与聚集诱导的发射(AIE)特性和近红外(NIR)的固态发射用于原位实时跟踪MMP变化。阳离子探针CSP仅用正常MMP染色线粒体。一旦MMP降低,CSP就可以从线粒体中释放出来并迁移到溶酶体中。随着MMP,CSP的恢复可以回到线粒体。有趣的是,CSP与商业溶酶体特异性染料(LB-NIR )之间的平均共定位系数(CLC)已成功用作实时跟踪MMP动态变化的可靠且实用的MMP参数。此外,AIEgens的其他独特功能,例如大斯托克斯位移(197 nm),高荧光量子产率(9.8%),出色的光稳定性和免洗生物成像,共同使CSP成为在线粒体中实时跟踪MMP的有前途的工具涉及病理生理过程。

更新日期:2020-10-04
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