Mitochondrial membrane potential (MMP) is a critical parameter representing mitochondrial function and cellular activities. The development of a robust and convenient method for real-time tracking the MMP changes is still highly desirable and urgently needed. Herein, we presented a novel mitochondria-lysosomes migration fluorescent probe (CSP) with aggregation-induced emission (AIE) characteristics and near-infrared (NIR) solid-state emission for in situ real-time tracking MMP variations. The cationic probe CSP exclusively stained mitochondria with normal MMP; once MMP decreases, CSP could be released from mitochondria and migrated to lysosomes; with the recovery of MMP, CSP could move back to the mitochondria. Interestingly, the average colocalization coefficient (CLC) between CSP and a commercial lysosome-specific dye (LB-NIR) have been successfully served as a reliable and practical MMP parameter for real-time tracking the dynamic changes of MMP. Moreover, other unique features of AIEgens such as large Stokes shift (197 nm), high fluorescence quantum yield (9.8 %), excellent photostability and wash-free bioimaging, together make CSP a promising tool for real-time tracking MMP during the mitochondria-involved pathophysiologic processes.

线粒体膜电位（MMP）是代表线粒体功能和细胞活性的关键参数。仍然非常需要并且迫切需要开发一种鲁棒且方便的方法来实时跟踪MMP变化。在此，我们提出了一个新颖的线粒体溶酶体移民工作荧光探针（CSP）与聚集诱导的发射（AIE）特性和近红外（NIR）的固态发射用于原位实时跟踪MMP变化。阳离子探针CSP仅用正常MMP染色线粒体。一旦MMP降低，CSP就可以从线粒体中释放出来并迁移到溶酶体中。随着MMP，CSP的恢复可以回到线粒体。有趣的是，CSP与商业溶酶体特异性染料（LB-NIR ）之间的平均共定位系数（CLC）已成功用作实时跟踪MMP动态变化的可靠且实用的MMP参数。此外，AIEgens的其他独特功能，例如大斯托克斯位移（197 nm），高荧光量子产率（9.8％），出色的光稳定性和免洗生物成像，共同使CSP成为在线粒体中实时跟踪MMP的有前途的工具涉及病理生理过程。