Transcriptome and metabolome profiling unveiled mechanisms of tea (Camellia sinensis) quality improvement by moderate drought on pre-harvest shoots,Phytochemistry

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Transcriptome and metabolome profiling unveiled mechanisms of tea (Camellia sinensis) quality improvement by moderate drought on pre-harvest shoots
Phytochemistry ( IF 3.2 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.phytochem.2020.112515
Meifeng Li ₁ , Jianjun Liu ₂ , Yuping Zhou ₃ , Siqin Zhou ₃ , Shuai Zhang ₃ , Huarong Tong ₁ , Aichun Zhao ₃

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Conventional wisdom holds that tea (Camellia sinensis) quality can be improved by drought. To clarify the underlying mechanism, a conjoint analysis of transcriptome and metabolome profiling was carried out in tea shoots harvested under different soil water contents (SWCs). Drought had little impact on theanine, catechins and caffeine in field conditions. Besides the flavor contributions of amino acid and their derivatives, organic acids, and nucleotides and their derivatives, the obviously increased isoflavonoids and glycosylflavonoids and the sharply decreased lipids are suggested to play key roles, which is mainly due to substantial increases of type III polyketide synthase B (PKSB), flavonol synthase/flavanone 3-hydroxylase (FLS), and UDP-glycosyltransferases (UGTs), as well as the significant repression of anthocyanidin synthase (ANS) and R2R3MYBs, and downregulated lipid metabolisms. Genes of GDSL esterase/lipase (GDSL), abscisic acid (ABA) and jasmonate (JA) signaling were found to play important roles in both flavonoid accumulation and lipid reduction. These findings increased our understanding of how moderate drought improves taste and aroma of tea by interfering in the metabolism of fresh leaves, which provides new insight into balancing compounds in pre-harvest tea shoots.

中文翻译：

转录组和代谢组分析揭示了中度干旱对采收前枝条茶（Camellia sinensis）品质改善的机制

传统观点认为，干旱可以改善茶（Camellia sinensis）的质量。为了阐明潜在机制，在不同土壤含水量 (SWC) 下收获的茶芽中进行了转录组和代谢组分析的联合分析。干旱对田间条件下的茶氨酸、儿茶素和咖啡因几乎没有影响。除了氨基酸及其衍生物、有机酸、核苷酸及其衍生物的风味贡献外，异黄酮和糖基黄酮的显着增加和脂质的急剧减少被认为起关键作用，这主要是由于III型聚酮合酶的显着增加B (PKSB)、黄酮醇合酶/黄烷酮 3-羟化酶 (FLS) 和 UDP-糖基转移酶 (UGT)，以及对花色素合成酶 (ANS) 和 R2R3MYBs 的显着抑制，以及下调脂质代谢。发现 GDSL 酯酶/脂肪酶 (GDSL)、脱落酸 (ABA) 和茉莉酸 (JA) 信号的基因在类黄酮积累和脂质减少中发挥重要作用。这些发现增加了我们对适度干旱如何通过干扰新鲜叶子的代谢来改善茶的味道和香气的理解，这为平衡采前茶芽中的化合物提供了新的见解。

更新日期：2020-12-01

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