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Fabrication and antibacterial activity of nanoenhanced conjugate of silver (I) oxide with graphene oxide
Materials Today Communications ( IF 3.7 ) Pub Date : 2020-09-21 , DOI: 10.1016/j.mtcomm.2020.101667
Afroz Khan , Fuad Ameen , Fauzia Khan , Abdullah Al-Arfaj , Bilal Ahmed

Due to the globally identified antibiotic resistance among clinical bacteria, novel antibacterial materials are needed to circumvent drug resistance. In this study, we report a facile synthesis method for the fabrication of a uniform silver (I) oxide (Ag2O) garnished graphene oxide (GO) nanocomposite (Ag2O/GO) using sonication and characterized them by X-ray diffraction (XRD), Raman spectroscopy, and UV–vis spectroscopy. Furthermore, the antibacterial properties of Ag2O, GO, and Ag2O/GO nanocomposite were studied using drug resistant Gram-negative Escherichia Coli (ESBL), Pseudomonas aeruginosa (ESBL), Klebsiella pneumoniae and Gram-positive Staphylococcus aureus by well diffusion assay, colony forming ability, and cell membrane permeability assay. The nanocomposite (15.62−1000 μg/mL) showed excellent antibacterial activity with minimum inhibitory concentration of 125 μg/mL for P. aeruginosa and K. pneumoniae; 62.5 μg/mL for E. coli, and 250 μg/mL for S. aureus. Biofilm inhibition assay revealed the inhibition of biofilm formation in a dose related manner. Inhibition of biofilm formation by ½ MIC and MIC of Ag2O and Ag2O/GO against four pathogenic and biofilm forming bacteria was found statistically significant as P ≤ 0.05 (at ½ MIC) and P ≤ 0.01 (at MIC). These results demonstrated that these facile nanomaterials are promising antibacterial and antibiofilm agents that can be utilized to inhibit the growth of human bacterial pathogens associated with chronic infections.



中文翻译:

氧化银(I)与氧化石墨烯的纳米增强共轭物的制备和抗菌活性

由于临床细菌中全球公认的抗生素耐药性,因此需要新型的抗菌材料来规避耐药性。在这项研究中,我们报告了一种通过超声处理制备均匀的氧化银(I)(Ag 2 O)装饰的氧化石墨烯(GO)纳米复合材料(Ag 2 O / GO)的简便合成方法,并通过X射线衍射对其进行了表征(XRD),拉曼光谱和紫外可见光谱。此外,使用耐药革兰氏阴性大肠杆菌(ESBL),铜绿假单胞菌(ESBL),肺炎克雷伯菌和革兰氏阳性研究了Ag 2 O,GO和Ag 2 O / GO纳米复合材料的抗菌性能。金黄色葡萄球菌通过孔扩散测定,集落形成能力和细胞膜通透性测定。纳米复合物(15.62-1000μg/ mL)具有优异的抗菌活性,对铜绿假单胞菌肺炎克雷伯菌的最低抑菌浓度为125μg/ mL ; 大肠杆菌为62.5μg/ mL,金黄色葡萄球菌为250μg/ mL 。生物膜抑制试验揭示了以剂量相关的方式抑制生物膜形成。Ag 2 O和Ag 2的½MIC和MIC抑制生物膜形成发现针对四种病原性和生物膜形成细菌的O / GO具有统计学意义,P≤0.05(在½MIC时)和P≤0.01(在MIC时)。这些结果表明,这些易用的纳米材料是有前途的抗菌和抗生物膜剂,可用于抑制与慢性感染有关的人类细菌病原体的生长。

更新日期:2020-09-21
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