Due to the globally identified antibiotic resistance among clinical bacteria, novel antibacterial materials are needed to circumvent drug resistance. In this study, we report a facile synthesis method for the fabrication of a uniform silver (I) oxide (Ag₂O) garnished graphene oxide (GO) nanocomposite (Ag₂O/GO) using sonication and characterized them by X-ray diffraction (XRD), Raman spectroscopy, and UV–vis spectroscopy. Furthermore, the antibacterial properties of Ag₂O, GO, and Ag₂O/GO nanocomposite were studied using drug resistant Gram-negative Escherichia Coli (ESBL), Pseudomonas aeruginosa (ESBL), Klebsiella pneumoniae and Gram-positive Staphylococcus aureus by well diffusion assay, colony forming ability, and cell membrane permeability assay. The nanocomposite (15.62−1000 μg/mL) showed excellent antibacterial activity with minimum inhibitory concentration of 125 μg/mL for P. aeruginosa and K. pneumoniae; 62.5 μg/mL for E. coli, and 250 μg/mL for S. aureus. Biofilm inhibition assay revealed the inhibition of biofilm formation in a dose related manner. Inhibition of biofilm formation by ½ MIC and MIC of Ag₂O and Ag₂O/GO against four pathogenic and biofilm forming bacteria was found statistically significant as P ≤ 0.05 (at ½ MIC) and P ≤ 0.01 (at MIC). These results demonstrated that these facile nanomaterials are promising antibacterial and antibiofilm agents that can be utilized to inhibit the growth of human bacterial pathogens associated with chronic infections.

由于临床细菌中全球公认的抗生素耐药性，因此需要新型的抗菌材料来规避耐药性。在这项研究中，我们报告了一种通过超声处理制备均匀的氧化银（I）（Ag ₂ O）装饰的氧化石墨烯（GO）纳米复合材料（Ag ₂ O / GO）的简便合成方法，并通过X射线衍射对其进行了表征（XRD），拉曼光谱和紫外可见光谱。此外，使用耐药革兰氏阴性大肠杆菌（ESBL），铜绿假单胞菌（ESBL），肺炎克雷伯菌和革兰氏阳性菌研究了Ag ₂ O，GO和Ag ₂ O / GO纳米复合材料的抗菌性能。金黄色葡萄球菌通过孔扩散测定，集落形成能力和细胞膜通透性测定。纳米复合物（15.62-1000μg/ mL）具有优异的抗菌活性，对铜绿假单胞菌和肺炎克雷伯菌的最低抑菌浓度为125μg/ mL ; 大肠杆菌为62.5μg/ mL，金黄色葡萄球菌为250μg/ mL 。生物膜抑制试验揭示了以剂量相关的方式抑制生物膜形成。Ag ₂ O和Ag _2的½MIC和MIC抑制生物膜形成发现针对四种病原性和生物膜形成细菌的O / GO具有统计学意义，P≤0.05（在½MIC时）和P≤0.01（在MIC时）。这些结果表明，这些易用的纳米材料是有前途的抗菌和抗生物膜剂，可用于抑制与慢性感染有关的人类细菌病原体的生长。