Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested.

寨卡病毒和登革热病毒具有相当大的免疫交叉反应性，由于出现假阳性结果而导致血清学诊断麻烦。由于巴西的循环黄病毒种类繁多，我们的目标是使用由巴西虫媒病毒国家参考实验室改编的内部血清学测试，并评估可用的商业测试。我们针对登革热、寨卡病毒、黄热病、罗西奥、伊列乌斯、圣路易斯脑炎、西尼罗河和基孔肯雅热呈阳性的一组样本，评估了内部 IgM ELISA 对抗 ZIKV、-DENV 和 -YFV IgM 的个体检测。我们还评估了 2015 年巴西卫生部推荐的两种用于登革热和寨卡病毒 IgM 检测的商业试剂盒。 内部 ZIKV IgM ELISA 的灵敏度和特异性分别为 60.0% 和 88%。6% 和内部 DENV IgM ELISA 分别为 100% 和 82.2%。内部 YFV IgM ELISA 的灵敏度和特异性均为 100%。Novagnost Zika Virus IgM 测试的灵敏度为 47.3%，特异性为 85.3%，Serion ELISA 经典登革病毒 IgM 分别为 92.8% 和 58.9%。总的来说，ZIKV 和 DENV 的内部 ELISA 在这里进行了调整和评估，表现出比测试的商业试剂盒更好的性能。