In the present study, the adsorption of phenolic compounds, first of all, chlorogenic acid isomers (chlorogenic, neo-chlorogenic and crypto-chlorogenic acids) predominant in the artichoke (AE) or green coffee bean (GCBE) extracts on cross-linked cationic starch having quaternary ammonium groups (CCS) has been investigated. The equilibrium adsorption studies showed that adsorption closely followed the Langmuir adsorption model, i.e. anionic substances of the extracts were interacting with quaternary ammonium groups of CCS. The UPLC-UV-MS/MS analysis showed that 8% and 17% of chlorogenic acid isomers of the total amount of adsorbed phenolics form AE and GCBE, respectively, were immobilized on CCS. The desorption study of phenolics from AE/CCS and GCBE/CCS complexes revealed that amount of desorbed AE or GCBE phenolics depended on the desorption medium. The antioxidant activity investigation showed that the immobilization of active components of extracts on CCS prevented the rapid loss of antioxidant activity. The results suppose that adsorption on modified starch technique can be successfully employed to remove important amounts of bioactive compounds from plant extracts by employing effective, sustainable and environmental friendly procedures.

在本研究中，首先在朝鲜蓟（AE）或生咖啡豆（GCBE）提取物中，主要在交联阳离子上吸附酚类化合物，首先是绿原酸异构体（绿原酸，新绿原酸和隐绿原酸）已经研究了具有季铵基团（CCS）的淀粉。平衡吸附研究表明，吸附紧随Langmuir吸附模型，即提取物中的阴离子物质与CCS的季铵基团相互作用。UPLC-UV-MS / MS分析表明，分别由AE和GCBE吸附的酚类总量的8％和17％的绿原酸异构体被固定在CCS上。从AE / CCS和GCBE / CCS络合物中解吸酚类物质的研究表明，解吸的AE或GCBE酚类物质的量取决于解吸介质。抗氧化活性研究表明，将提取物的活性成分固定在CCS上可以防止抗氧化活性的快速丧失。结果认为，通过采用有效，可持续和环境友好的程序，改性淀粉技术上的吸附可以成功地从植物提取物中去除重要量的生物活性化合物。