Vaccination is one of the strategies for preventing Vibrio harveyi infection in marine-cultured animals. In this study, we prepared a formalin-killed cells of V. harveyi ZJ0603 vaccine (FKC) combined with β-glucan to immune pearl gentian grouper. The results indicated that the expression levels of IgM, TNF-α, MHC-Iα, IL-1β and IL-16 significantly increased in the spleen of the vaccinated fish. Antibody titers, activities of lysozyme and superoxide dismutase were significantly prompted in blood of the vaccinated fish. After 35 d post-vaccination, all fish were challenged intraperitoneally by virulent V. harveyi, and the relative percentage of survival (RPS) of FKC+β-glucan, FKC, β-glucan and PBS were 68 ± 5.7%, 55 ± 8.5%, 42 ± 7.5% and 32 ± 6.9%, respectively. These results demonstrated that β-glucan could be as a potential adjuvant of FKC and provide good protective effect against V. harveyi infection in the pearl gentian grouper culture.

疫苗接种是预防海洋养殖动物中哈维弧菌感染的策略之一。在这项研究中，我们制备了福尔马林杀死哈维氏弧菌ZJ0603疫苗（FKC）与β-葡聚糖相结合的细胞，以免疫珍珠龙胆石斑鱼。结果表明，接种鱼的脾脏中IgM，TNF-α，MHC-Iα，IL-1β和IL-16的表达水平显着增加。接种鱼的血液中显着促进了抗体滴度，溶菌酶和超氧化物歧化酶的活性。疫苗接种35天后，所有鱼类均受到强毒哈氏弧菌腹膜内攻击，FKC +β-葡聚糖，FKC，β-葡聚糖和PBS的相对生存率（RPS）分别为68±5.7％，55±8.5％，42±7.5％和32±6.9​​％。这些结果表明，β-葡聚糖可以作为FKC的潜在佐剂，并在珍珠龙胆石斑鱼培养物中提供对哈维氏弧菌感染的良好保护作用。