A metagenome-wide association study of gut microbiome and visceral fat accumulation,Computational and Structural Biotechnology Journal

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A metagenome-wide association study of gut microbiome and visceral fat accumulation
Computational and Structural Biotechnology Journal ( IF 4.4 ) Pub Date : 2020-09-20 , DOI: 10.1016/j.csbj.2020.09.026
Xiaomin Nie ₁ , Jiarui Chen _{2,

3} , Xiaojing Ma ₁ , Yueqiong Ni ₂ , Yun Shen ₁ , Haoyong Yu ₁ , Gianni Panagiotou _{2,

3,

4} , Yuqian Bao ₁

Affiliation

Purpose

Visceral fat is an independent risk factor for metabolic and cardiovascular disease. The study aimed to investigate the associations between gut microbiome and visceral fat.

Methods

We recruited 32 obese adults and 30 healthy controls at baseline. Among the obese subjects, 14 subjects underwent laparoscopic sleeve gastrectomy (LSG) and were followed 6 months after surgery. Abdominal visceral fat area (VFA) and subcutaneous fat area (SFA) were measured by magnetic resonance imaging. Waist, hipline, waist-to-hip ratio (WHR) and body mass index (BMI) were included as simple obese parameters. Gut microbiome was analyzed by metagenomic sequencing.

Results

Among the obese parameters, VFA had the largest number of correlations with the species that were differentially enriched between obese and healthy subjects, following by waist, WHR, BMI, hipline, and SFA. Within the species negatively correlated with VFA, Eubacterium eligens had the strongest correlation, following by Clostridium citroniae, C. symbiosum, Bacteroides uniformis, E. ventriosum, Ruminococcaceae bacterium D16, C. hathewayi, etc. C. hathewayi and C. citroniae were increased after LSG. Functional analyses showed that among all the obese parameters, VFA had strongest correlation coefficients with the obesity-related microbial pathways. Microbial pathways involved in carbohydrate fermentation and biosynthesis of L-glutamate and L-glutamine might contribute to visceral fat accumulation.

Conclusions

Visceral fat was more closely correlated with gut microbiome compared with subcutaneous fat, suggesting an intrinsic connection between gut microbiome and metabolic cardiovascular diseases. Specific microbial species and pathways which were closely associated with visceral fat accumulation might contribute to new targeted therapies for metabolic disorders.

中文翻译：

肠道微生物组和内脏脂肪积累的宏基因组关联研究

目的

内脏脂肪是代谢和心血管疾病的独立危险因素。该研究旨在调查肠道微生物组与内脏脂肪之间的关联。

方法

我们在基线时招募了 32 名肥胖成人和 30 名健康对照。在肥胖受试者中，14 名受试者接受了腹腔镜袖状胃切除术 (LSG)，并在术后 6 个月进行了随访。通过磁共振成像测量腹部内脏脂肪面积（VFA）和皮下脂肪面积（SFA）。腰围、臀围、腰臀比（WHR）和体重指数（BMI）作为简单的肥胖参数包括在内。通过宏基因组测序分析肠道微生物组。

结果

在肥胖参数中，VFA与肥胖和健康受试者之间差异丰富的物种的相关性最多，其次是腰围、WHR、BMI、臀线和SFA。在与VFA负相关的物种中，Eubacterium eligens的相关性最强，其次是Clostridium citroniae、C. symbiosum、Bacteroides uniformis、E. ventriosum、瘤胃球菌D16、C. hathewayi等。C. hathewayi和C. citroniaeLSG 后增加。功能分析表明，在所有肥胖参数中，VFA 与肥胖相关微生物途径的相关系数最强。参与碳水化合物发酵和 L-谷氨酸和 L-谷氨酰胺生物合成的微生物途径可能有助于内脏脂肪的积累。