The microbial consortia designedly screened in our lab were used for solid-state pretreatment of tree trimmings to degrade lignin selectively in this work, and the commercial fungal isolate pretreatment was taken in comparison. The results showed that a highly selective degradation of lignin was observed in the tree trimmings pretreated via the microbial consortium DM-1, in which 14.0% of lignin was decomposed and no significant degradation of cellulose was noted within 20 days. Meanwhile, in this trial, shorter fibers and a lower crude fiber crystallinity were found in the tree trimmings after pretreatment. And the pH values of the tree trimmings undergoing biopretreatment in each trial were all kept at around 7.0. In the SSF (simultaneous saccharification and fermentation) process, 20% increment of the ethanol yield was obtained in the trial loaded with DM-1 pretreated tree trimmings compared to the control, in which a higher acid-based buffering capability was also observed. The scanning electron microscope test revealed that after SSF, the DM-1 pretreated tree trimmings had less crude fibers and more void ratios. Herein, it was feasible to produce more ethanol from tree trimmings via solid-state pretreatment by using the microbial consortium DM-1.

在我们的实验室中设计筛选的微生物联盟在这项工作中用于树木装饰物的固态预处理，以选择性地降解木质素，并进行了商业真菌分离预处理的比较。结果表明，在通过微生物联合会DM-1预处理的树木装饰物中观察到了木质素的高度选择性降解，其中木质素14.0％被分解，并且在20天内未观察到纤维素的显着降解。同时，在该试验中，预处理后的树木装饰物中发现了较短的纤维和较低的粗纤维结晶度。在每个试验中，经过生物预处理的装饰物的pH值均保持在7.0左右。在SSF（同时糖化和发酵）过程中，与对照相比，在装有DM-1预处理树木修剪物的试验中，乙醇产量增加了20％，在该试验中还观察到了更高的基于酸的缓冲能力。扫描电子显微镜测试显示，经过SSF处理后，DM-1预处理的树木装饰物的粗纤维更少，空隙率更高。在此，通过使用微生物聚生体DM-1通过固态预处理从树木修剪产生更多的乙醇是可行的。