The Journal of Membrane Biology ( IF 2.3 ) Pub Date : 2020-09-21 , DOI: 10.1007/s00232-020-00137-y Natalia V Ozolina 1 , Irina S Nesterkina 1 , Veronika V Gurina 1 , Vadim N Nurminsky 1
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Abstract
Vacuolar and plasma membranes were isolated by a detergent-free method from beet roots (Beta vulgaris L.), and were fractionated in a sucrose density gradient of 15–60% by high-speed centrifugation at 200,000×g during 18 h. The membrane material distributed over the sucrose density gradient was analyzed for the presence of lipids characteristic of raft structures in different zones of the gradient. The quantitative and qualitative content of lipids and sterols, and the composition of fatty acids were analyzed. Some membrane structures differing in their biochemical characteristics were revealed to be located in different zones of the sucrose gradient. The results of the analysis allowed us to identify three zones in the sucrose gradient after the vacuolar membrane fractionation and two zones in the plasma membrane where membrane structures, which may be defined as rafts for their lipid composition, were presented.
Graphic Abstract
中文翻译：
从甜菜浆和液泡膜中分离膜结构的非清洁剂具有筏的脂质组成特征。
摘要
通过无洗涤剂方法从甜菜根 ( Beta vulgaris L.)中分离液泡膜和质膜，并通过 200,000× g高速离心以 15-60% 的蔗糖密度梯度进行分级在 18 小时内。分析分布在蔗糖密度梯度上的膜材料，以确定梯度不同区域中筏结构特征的脂质的存在。分析了脂质和甾醇的定量和定性含量，以及脂肪酸的组成。一些生物化学特性不同的膜结构被揭示位于蔗糖梯度的不同区域。分析结果使我们能够在液泡膜分离后确定蔗糖梯度中的三个区域和质膜中的两个区域，其中存在膜结构，这些区域可以定义为它们的脂质组成的筏子。
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