A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l^{− 1} benzyladenine and 0.5 mg l^{− 1} indole acetic acid. The effect of the bioreactor type (RITA® and Plantform™), frequency of the immersions, regulator concentrations, and volume of medium per explant was investigated. All the treatments with the temporal immersion systems (TIS) increased the proliferation rates. The best results were obtained on using the Plantform™ vessels and, unlike the shoots cultured in RITA® vessels, the culture in Plantform™ had very low hyperhydricity percentages. Shoots originating from the culture in semi-solid medium and in Plantform™ rooted in semi-solid medium with 0.1 mg/l indole butyric acid for 7 days. No significant differences were observed in the rooting or acclimatization percentages, with survival percentages greater than 85% being achieved. This is the first work on the use of TIS systems in alder propagation, with the results of this study providing new perspectives for its mass propagation.

已经开发了用于在液体培养基中繁殖Al木腋生芽的方案。将外植体在木本植物培养基中培养在补充有0.1毫克升^{- 1个}苄基腺嘌呤和0.5毫克升^{- 1}吲哚乙酸。研究了生物反应器类型（RITA®和Plantform™），浸入频率，调节剂浓度和每个外植体培养基体积的影响。暂时浸入系统（TIS）的所有处理均增加了增殖率。使用Plantform™容器可获得最佳结果，与在RITA®容器中培养的芽不同，Plantform™中的培养物的超水百分比非常低。源于半固体培养基中的植物和以0.1 mg / l吲哚丁酸在半固体培养基中生根的Plantform™7天的芽。生根或驯化百分比没有观察到显着差异，存活百分比大于85％。这是在al木传播中使用TIS系统的第一项工作，