Abstract

Dunaliella salina (D. salina) has been widely applied in various fields because of its inherent advantages, such as the study of halotolerant mechanism, wastewater treatment, recombinant proteins expression, biofuel production, preparation of natural materials, and others. However, owing to the existence of low yield or in the laboratory exploration stage, D. salina system has been greatly restricted for practical production of various components. In past decade, significant progresses have been achieved for research of D. salina in these fields. Among them, D. salina as a novel expression system demonstrated a bright prospect, especially for large-scale production of foreign proteins, like the vaccines, antibodies, and other therapeutic proteins. Due to the low efficiency, application of traditional regulation tools is also greatly limited for exploration of D. salina system. The emergence of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system offers a precise editing tool to overcome the obstacles of D. salina system. This review not only comprehensively summarizes the recent progresses of D. salina in domain of gene engineering but also gives a deep analysis of problems and deficiencies in different fields of D. salina. Moreover, further prospects of CRISPR/Cas system and its significant challenges have been discussed in various aspects of D. salina. It provides a great referencing value for speeding up the maturity of D. salina system, and also supplies practical guiding significance to expand the new application fields for D. salina.

Key points

• The review provides recent research progresses of various applications of D. salina.

• The problems and deficiencies in different fields of D. salina were deeply analyzed.

• The further prospects of CRISPR/Cas technology in D. salina system were predicted.

• CRISPR/Cas system will promote the new application fields and maturity for D. salina.

中文翻译：

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CRISPR / Cas技术促进了杜氏藻盐沼系统的各种应用。

摘要

杜氏盐藻（D. salina）（D. salina）由于其固有的优势而被广泛应用于各个领域，例如研究卤化钾机理，废水处理，重组蛋白表达，生物燃料生产，天然材料的制备等。然而，由于存在低产量或在实验室探索阶段，盐沼假单胞菌系统已严重限制了各种组分的实际生产。在过去的十年中，在这些领域中盐藻的研究取得了重大进展。其中，D。salina新型表达系统的应用前景广阔，特别是大规模生产外源蛋白，如疫苗，抗体和其他治疗性蛋白。由于效率低下，传统调节工具的应用也大大限制了盐藻的探索。簇状规则间隔的短回文重复序列（CRISPR）/ Cas系统的出现提供了一种精确的编辑工具，可克服盐藻D. salina系统的障碍。这篇综述不仅全面总结了盐沼石gene在基因工程领域的最新进展，而且对盐沼石different不同领域的问题和不足进行了深入的分析。。此外，在D. salina的各个方面都讨论了CRISPR / Cas系统的进一步前景及其重大挑战。它为加快盐沼系统的成熟提供了很好的参考价值，也为拓展盐沼的新应用领域提供了实际的指导意义。

关键点

•综述提供了盐藻的各种应用的最新研究进展。

•深入分析了盐藻的各个领域存在的问题和不足。

•预测了D. salina系统中CRISPR / Cas技术的进一步前景。

•CRISPR / Cas系统将促进D. salina的新应用领域和成熟度。