Plants possess the structural maintenance of chromosome (SMC) protein complexes cohesin, condensin, and SMC5/6, which function in fundamental biological processes such as sister chromatid cohesion, chromosome condensation and segregation, and damaged DNA repair. Recently, increasing evidence in several organisms has suggested that condensin is involved in chromatin organizations during interphase. In Arabidopsis thaliana, condensin II is localized in the nucleus throughout interphase and is suggested to be required for keeping centromeres apart and the assembly of euchromatic chromosome arms. However, it remains unclear how condensin II organizes chromatin associations. Here, we first showed the high possibility that the function of condensin II as a complex is required for the disassociation of centromeres. Analysis of the rDNA array distribution revealed that condensin II is also indispensable for the association of centromeres with rDNA arrays. Reduced axial compaction of chromosomes and impaired genome integrity in condensin II mutants are not related to the disruption of chromatin organization. In contrast, the axial compaction of chromosomes by condensin II produces the force leading to the disassociation of heterologous centromeres in Drosophila melanogaster. Taken together, our data imply that the condensin II function in chromatin organization differs among eukaryotes.

植物具有染色体（SMC）蛋白复合物粘着素，凝缩蛋白和SMC5 / 6的结构维持功能，它们在基本的生物过程中起作用，例如姐妹染色单体的粘着，染色体凝结和分离以及受损的DNA修复。最近，越来越多的生物证据表明凝缩蛋白在相间参与了染色质的组织。在拟南芥中，condensin II位于整个相间的细胞核中，建议将其用于保持着丝粒分离和常染色体染色体臂的组装。然而，目前尚不清楚condensin II如何组织染色质缔合。在这里，我们首先显示了凝集素II作为复合物的功能对于着丝粒解离而言具有很高的可能性。对rDNA阵列分布的分析表明，凝聚素II对于着丝粒与rDNA阵列的结合也是必不可少的。condensin II突变体中染色体轴向紧密度降低和基因组完整性受损与染色质组织破坏无关。相反，凝缩蛋白II对染色体的轴向压紧产生了导致异源着丝粒解离的力。果蝇。两者合计，我们的数据表明，在真核生物之间，染色质组织中的凝聚素II功能有所不同。