This study assessed the effect of poly‐β‐hydroxybutyrate (PHB) on the growth, survival, and disease resistance of Pacific white shrimp Litopenaeus vannamei. A 20‐d feeding test using 30‐d‐postlarval Pacific white shrimp (100 shrimp/tank) tested three different diets: 0.3 g PHB/kg diet, 0.5 g PHB/kg diet, and commercial feed containing no PHB as the control. The feeding test was followed by a 10‐d bacterial challenge test using Vibrio harveyi. After the feeding test, the highest average final body weight, specific growth rate, and total biomass (mean ± SD = 0.44 ± 0.28 g, 28.82 ± 2.08%/d, and 25.91 ± 7.19 g, respectively) were found in the 0.5 g PHB/kg diet group, while the 0.3 g PHB/kg diet showed the highest survival rate (67.00 ± 8.83%). After the Vibrio harveyi challenge test, both PHB diet treatments resulted in the same significantly higher survival rate (96.66 ± 4.71%) compared with the control treatment (70.00 ± 4.71%). The highest body weight gain during the challenge test was seen in the 0.5 g PHB/kg diet group. Total abundance of heterotrophic bacteria and Vibrio spp. was relatively similar for all treatments. Histopathological analysis showed that hepatopancreas samples from PHB‐supplemented Pacific white shrimp showed decreasing tubular epithelial cell lesions as a result of Vibrio harveyi infection, suggesting that PHB protected the hepatopancreas of Pacific white shrimp from the destructive effect of Vibrio harveyi infection. Supplementation at 0.5 g PHB/kg significantly increased Pacific white shrimp survival and growth with or without Vibrio harveyi infection and, thus, has high potential for further use in Pacific white shrimp aquaculture.

中文翻译：

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聚-β-羟基丁酸酯（PHB）改善苗圃期太平洋白对虾凡纳滨对虾对弧菌病的防御

这项研究评估了聚-β-羟基丁酸酯（PHB）对南美白对虾凡纳滨对虾的生长，存活和抗病性的影响。使用30 d幼体期后的太平洋白虾（100虾/罐）进行的20 d饲喂试验测试了三种不同的日粮：0.3 g PHB / kg日粮，0.5 g PHB / kg日粮和不含PHB的商业饲料作为对照。喂食测试后，使用哈维弧菌进行10天细菌攻击测试。喂食测试后，在0.5克中发现最高的平均最终体重，比生长率和总生物量（分别为平均值±SD = 0.44±0.28 g，28.82±2.08％/ d和25.91±7.19 g）。 PHB / kg饮食组，而0.3 g PHB / kg饮食显示最高的生存率（67.00±8.83％）。后哈维氏弧菌挑战试验中，两种PHB饮食治疗均比对照组（70.00±4.71％）的存活率显着提高（96.66±4.71％）。在0.5 g PHB / kg饮食组中，挑战试验期间体重增加最高。异养细菌和弧菌属菌种的总丰度。所有治疗方法都相对相似。组织病理学分析表明，由于哈维氏弧菌感染，PHB补充的太平洋白对虾的肝胰腺样本显示肾小管上皮细胞病变减少，这表明PHB保护太平洋白对虾的肝胰腺免受哈维氏弧菌的破坏作用感染。以0.5 g PHB / kg的剂量添加，无论有无哈维弧菌感染，均能显着提高太平洋白对虾的存活和生长，因此，在太平洋白对虾养殖中具有很高的应用潜力。