ABSTRACT A high-density linkage map was constructed for mānuka (Leptospermum scoparium) genotyping by sequencing (GBS) and a segregating population developed using accessions from the East Cape region of New Zealand. Parental maps were constructed using 1140 and 1509 SNPs markers grouped into 11 linkage groups for both parents spanning a total of 1242.8 and 1616.2 cM, respectively. Quantitative trait loci (QTL) analysis was performed for tree height. In total, 10 QTLs were detected after the measured variable was adjusted for the block effect in the field. The significant QTLs were inherited from both parents (five each) and were located on LG2, 3, 6, 7, 9 and 10. This is the first high-density linkage map constructed using thousands of genetic markers obtained by GBS, the first QTL reported for a New Zealand native tree species, which represents a model for future genetic analysis of indigenous New Zealand species.

中文翻译：

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新西兰 mānuka (Leptospermum scoparium L.) 树木生长的高密度连锁图和数量性状位点

摘要构建了高密度连锁图谱，用于通过测序 (GBS) 进行 mānuka (Leptospermum scoparium) 基因分型和使用新西兰东开普地区的种质开发的分离种群。亲本图谱是使用 1140 和 1509 个 SNP 标记构建的，这些标记分为 11 个连锁组，父母双方的总长度分别为 1242.8 和 1616.2 cM。对树高进行数量性状基因座（QTL）分析。根据田间块效应调整测量变量后，总共检测到10个QTL。显着性 QTL 分别遗传自父母双方（各 5 个），位于 LG2、3、6、7、9 和 10。这是第一个利用 GBS 获得的数千个遗传标记构建的高密度连锁图，第一个 QTL报告了新西兰本地树种，