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A DNA barcode survey of marine macroalgae from Bergen (Norway)
Marine Biology Research ( IF 1.1 ) Pub Date : 2019-12-13 , DOI: 10.1080/17451000.2019.1699659
Trevor T. Bringloe 1, 2 , Kjersti Sjøtun 3 , Gary W. Saunders 1
Affiliation  

Safeguarding the services provided by marine forests inherently depends on an accurate understanding of macroalgal species diversity. Here, we provide the first DNA barcode survey of marine macroalgae from Norway, with a focus on the Bergen area, and compared our findings to morphological listings for the corresponding location. Specimens were sampled 14–20 April and 3–13 June 2016, and variously sequenced for several genetic markers, including the five prime end of the cytochrome c oxidase subunit I gene (COI-5P), elongation factor tufA, and the ribulose-1, 5-biphosphate carboxylase large subunit gene (rbcL). We generated 655 new barcode records for COI-5P, 11 for tufA, and 50 for rbcL, representing nine species of Chlorophyta, 51 species of Phaeophyceae, and 74 species of Rhodophyta. Sequence data confirmed the presence of 113 morphological species. A further 17 genetic groups indicated the presence of new species for the area, only six of which were linked to formally described species. The remaining four genetic records were uncertain in terms of morphological species assignment and relation to the local flora. We recommend further DNA barcoding surveys in the area, as only a third of the listed morphological species were genetically confirmed.



中文翻译:

来自卑尔根(挪威)的海洋大型藻类的DNA条码调查

维护海洋森林提供的服务本质上取决于对大型藻类多样性的准确了解。在这里,我们提供了挪威海洋藻类的首次DNA条码调查,重点是卑尔根地区,并将我们的发现与相应位置的形态列表进行了比较。样本于2016年4月14日至20日和6月3日至13日进行了采样,并对几种遗传标记进行了测序,包括细胞色素C氧化酶亚基I基因(COI-5P)的五个主要末端,延伸因子tufA和核糖1 ,5-二磷酸羧化酶大亚基基因(rbc L)。我们生成COI-5P 655条新的条形码记录,11的tufA和50 RBCL,代表叶绿藻的9种,藻科的51种和红藻的74种。序列数据证实了113种形态学物种的存在。另有17个遗传群体表明该地区存在新物种,其中只有6个与正式描述的物种有关。其余四个遗传记录在形态学物种分配和与当地菌群的关系方面尚不确定。我们建议对该区域进行进一步的DNA条码调查,因为仅列出的形态学物种中的三分之一已通过遗传学证实。

更新日期:2019-12-13
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