Rice blast, caused by the fungal pathogen Magnaporthe oryzae (M. oryzae), is one of the most destructive and widespread plant diseases in the world. Utilization of resistance genes in rice breeding is considered to be an effective and economical method to control this disease. To identify new sources of blast resistance, a set of 1160 introgression lines (ILs) containing chromosome segments of Chaling common wild rice (Oryza rufipogon Griff.) in the genetic background of an elite indica rice variety 93‐11 were developed and phenotyped in the blast nursery. Thirty‐three ILs displaying stable blast resistance in three consecutive years were obtained. Among them, one line, IL1043, was subsequently found to be resistant to all of the 28 M. oryzae isolates from different regions through artificial inoculation in greenhouse. By combining bulk segregant analysis coupled with next‐generation sequencing (BSA‐seq) and recessive class analysis (RCA), a major blast resistance gene in IL1043, designated Picl(t), was mapped on rice chromosome 6 flanked by the markers RM527 and Indel6 with an interval of approximately 925 kb, which covers the Pi2/9 locus. These results will facilitate fine mapping and cloning of Picl(t), and the linked markers will further provide a useful tool for rice blast resistance breeding.

中文翻译：

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茶岭普通野生稻(Oryza rufipogon Griff.)抗稻瘟病基因Picl(t)的鉴定

稻瘟病是由真菌病原体 Magnaporthe oryzae (M. oryzae) 引起的，是世界上最具破坏性和最普遍的植物病害之一。在水稻育种中利用抗性基因被认为是控制该病害的有效且经济的方法。为了鉴定抗稻瘟病的新来源，在优良籼稻品种 93-11 的遗传背景中开发了一组 1160 个基因渗入系（ILs），其中包含茶岭普通野生稻（Oryza rufipogon Griff.）的染色体片段。爆炸苗圃。获得了连续三年表现出稳定抗爆性的 33 个 IL。其中，IL1043 株系随后通过温室人工接种被发现对来自不同地区的所有 28 株米曲霉分离株均具有抗性。通过将批量分离分析与下一代测序 (BSA-seq) 和隐性类别分析 (RCA) 相结合，IL1043 中的一个主要稻瘟抗性基因 Picl(t) 被定位在水稻 6 号染色体上，两侧是标记 RM527 和Indel6 的间隔约为 925 kb，覆盖了 Pi2/9 基因座。这些结果将有助于Picl(t)的精细定位和克隆，连锁标记将进一步为稻瘟病抗性育种提供有用的工具。