We isolated and characterized a total of 13 microsatellite loci from Cordulegaster sarracenia (Odonata: Cordulegastridae). Loci were screened in 24 individuals from Louisiana and Texas. Within C. sarracenia, the number of alleles per locus ranged from 0 to 5, and observed and expected heterozygosities ranged from 0.000 to 0.556 and 0.000 to 0.613, respectively. Overall differentiation among study populations was very high (FST = 0.423), suggesting significant geographic population structure with low diversity within populations. Twelve of the 13 primers amplified in C. sayi, C. diastatops, C. maculata, and C. obliqua and polymorphism levels are reported. These new genetic markers will provide tools for addressing a number of population genetic and demographic questions relating to conservation of this rare dragonfly species.

中文翻译：

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一种稀有蜻蜓 Cordulegaster sarracenia（蜻蜓目：Cordulegastridae）的多态微卫星标记的开发，以及种群结构和遗传多样性的说明

我们从 Cordulegaster sarracenia (Odonata: Cordulegastridae) 中分离并表征了总共 13 个微卫星位点。对来自路易斯安那州和德克萨斯州的 24 个人进行了基因座筛选。在 C. sarracenia 中，每个基因座的等位基因数量范围为 0 到 5，观察到的和预期的杂合度范围分别为 0.000 到 0.556 和 0.000 到 0.613。研究人群之间的总体分化非常高（FST = 0.423），表明人口地理结构显着，种群内的多样性较低。报告了在 C.sayi、C.diastatops、C.maculata 和 C.obliqua 中扩增的 13 个引物中的 12 个，以及多态性水平。这些新的遗传标记将为解决与保护这种稀有蜻蜓物种有关的许多种群遗传和人口统计问题提供工具。