Abstract Calcium distribution in fertile and sterile anthers of a thermosensitive male-sterile wheat genotype was investigated using an antimonate precipitation method. During fertile anther development, before meiosis of the microspore mother cells, calcium precipitates were apparent in tapetal cells of the anther wall. After meiosis, precipitates were detected in the early microspores and accumulated in the large vacuole of late microspores. After microspore division, following decomposition of the large vacuole, precipitates decreased in the bicellular pollen. The earliest abnormality in calcium precipitate distribution detected during sterile pollen development was the greater accumulation of precipitates in the cytoplasm and nucleus of late microspores. The sterile microspore can divide to form bicellular pollen, but the large vacuole of sterile bicellular pollen did not decompose and greater abundance of precipitates was retained in the large vacuole. Abnormal distribution of calcium precipitates in sterile pollen precedes structural changes, suggesting that abnormal calcium metabolism is associated with pollen abortion.

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热敏雄性不育小麦可育和不育花药中钙的分布

摘要 使用锑酸盐沉淀法研究了热敏性雄性不育小麦基因型的可育和不育花药中钙的分布。在可育的花药发育过程中，在小孢子母细胞减数分裂之前，在花药壁的绒毡层细胞中有明显的钙沉淀。减数分裂后，在早期小孢子中检测到沉淀物，并在晚期小孢子的大液泡中积累。小孢子分裂后，随着大液泡的分解，双细胞花粉中的沉淀物减少。在不育花粉发育过程中检测到的钙沉淀分布的最早异常是晚期小孢子的细胞质和细胞核中沉淀的更大积累。不育的小孢子可以分裂形成双细胞花粉，但不育双细胞花粉的大液泡没有分解，大液泡中保留了更多的沉淀物。不育花粉中钙沉淀的异常分布先于结构变化，表明钙代谢异常与花粉败育有关。