Abstract Microsporongium development in Chinese fir (Cunninghamia lanceolata) was investigated using cytochemical methods with a special attention to the fluctuations (in amount and distribution) of polysaccharide and lipid reserves along the development of the microsporangium. Semi-thin sections of microsporangia at different developmental stages were stained with periodic acid–Schiff (PAS) reagent and Sudan Black B to detect insoluble polysaccharides and neutral lipids, respectively. In young microsporangia, microspore mother cells began to accumulate starch grains and lipids, which disappeared during microspore development. Following microspore division, the starch grains present in bicellular pollen disappeared and abundant lipid deposits were accumulated. In mature pollen, only abundant lipids accumulated as storage material. The pollen wall of C. lanceolata is predominantly composed of polysaccharidic intine, and the sporopollenin-containing exine is weakly developed and only forms a thin layer covering the intine.

中文翻译：

---

杉木发育中小孢子囊中多糖和脂质的动态变化

摘要 使用细胞化学方法研究了杉木 (Cunninghamia lanceolata) 中小孢子囊的发育，特别关注了随着小孢子囊发育的多糖和脂质储备的波动（数量和分布）。不同发育阶段的小孢子囊半薄切片用高碘酸-希夫（PAS）试剂和苏丹黑 B 染色，分别检测不溶性多糖和中性脂质。在年轻的小孢子囊中，小孢子母细胞开始积累淀粉粒和脂质，这些淀粉粒和脂质在小孢子发育过程中消失。小孢子分裂后，双细胞花粉中的淀粉粒消失，积累了大量的脂质沉积物。在成熟的花粉中，只有丰富的脂质积累作为储存物质。