Leaf yellowing symptoms were observed on Acacia mangium in the Sipahijala district of Tripura, India, during June 2017. Symptomatic and asymptomatic leaf samples (three of each) were collected from roadside trees of A. mangium for DNA extraction using the CTAB method. Amplicons of ~1.25 kb and ~480 bp were detected in all the symptomatic samples using the phytoplasma‐specific universal 16S rRNA and secA gene primers. Pair wise sequence analysis of 16S rRNA gene sequences, virtual RFLP and phylogenetic analysis revealed that the phytoplasma strain associated with A. mangium belonged to phytoplasma subgroup 16SrII‐C. This is the first report of an association between the 16SrII‐C subgroup and A. mangium leaf yellowing.

中文翻译：

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印度特里普拉邦 16Sr II-C 亚群植原体与 Acacia mangium 关联的第一份报告

2017 年 6 月，在印度特里普拉邦 Sipahijala 区的 Acacia mangium 上观察到叶子变黄症状。 从路边的 A. mangium 树中收集有症状和无症状的叶子样本（各三个），用于使用 CTAB 方法提取 DNA。使用植原体特异性通用 16S rRNA 和 secA 基因引物在所有有症状的样本中检测到 ~1.25 kb 和 ~480 bp 的扩增子。16S rRNA 基因序列的配对序列分析、虚拟 RFLP 和系统发育分析表明，与 A. mangium 相关的植原体菌株属于植原体亚群 16SrII-C。这是关于 16SrII-C 亚群与 A. mangium 叶子变黄之间关联的第一份报告。