Morphological, molecular and ecological studies revealed that Kiefferia Mik, 1985 (Diptera: Cecidomyiidae) is not a monotypic genus. Two new species, Kiefferia ezoensis sp. nov. and K. olla sp. nov. from Japan and Korea are added to the known European species, Kiefferia pericarpiicola. The new and known species are distinguishable from each other mainly by the shape of the larval sternal spatula. Molecular data support the results of morphological classification. Species of Kiefferia induce fruit galls on various plant species of Apiaceae. We found that K. ezoensis and K. olla utilized exclusively Angelica ursina and Oenanthe javanica, respectively. In contrast, we listed 37 apiaceous species belonging to 23 genera as hosts for K. pericarpiicola from previously published reports, suggesting the existence of additional undescribed species. Life history patterns of the three species are distinctly different from each other. Mature larvae of K. ezoensis and K. olla drop to the ground with the fruit gall in October and September, respectively, whereas mature larvae of K. pericarpiicola quit the galls and drop to the ground in August. A key to the Kiefferia species is provided based on morphological features and information on life history patterns and host ranges.

中文翻译：

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通过形态学、分子学和生态学研究检测 Kiefferia（双翅目：Cecidomyiidae）的两个新隐性物种

形态、分子和生态学研究表明 Kiefferia Mik，1985（双翅目：Cecidomyiidae）不是单型属。两个新物种 Kiefferia ezoensis sp。十一月 和 K. olla sp。十一月 来自日本和韩国的植物被添加到已知的欧洲物种 Kiefferia pericarpiicola 中。新物种和已知物种的区别主要在于幼虫胸骨刮刀的形状。分子数据支持形态学分类的结果。Kiefferia 的物种在伞形科的各种植物物种上诱导果瘿。我们发现 K. ezoensis 和 K. olla 分别专门使用了当归和 Oenanthe javanica。相比之下，我们从先前发表的报告中列出了属于 23 属的 37 种蜂类作为 K. pericarpiicola 的宿主，这表明存在其他未描述的物种。这三个物种的生活史模式彼此明显不同。K. ezoensis 和 K. olla 的成熟幼虫分别在 10 月和 9 月随果瘿下地，而 K. pericarpiicola 的成熟幼虫则在 8 月离开果瘿并下地。根据形态特征和生活史模式和宿主范围的信息，提供了 Kiefferia 物种的关键。