This study was conducted in the KVRS to; evaluate the seasonal eco-toxicity of water, soil and sediment samples; establish the suitability of using temperate biotest batteries in tropical systems; classify the toxicity of samples using Fuzzy Rules to estimate potential ecological risks. 143 water, sediment, and soil samples were collected during dry and rainy seasons in plantation areas. Pseudokirchneriella subcapitata, Aliivibrio fischeri and Arthrobacter globiformis bioassays were used to assess the toxicity of the samples. Results were categorized and classified into toxicity classes.

Dry season presented a significantly higher mean inhibition of 31% than 5% shown by rainy season samples (p < 0.001) in the bacterial bioassays, indicating a lower concentration of contaminants due to flooding and increased surface runoff. A few sediment samples resulted into 100% inhibition of A. globiformis, implying organisms were physiologically inactive upon exposure to contaminants. Seventy-three percent of samples posed little or no toxic potential risk, 25% posed critical risk and 1% posed elevated critical risk, implying the KVRS ecosystem might be at risk if the extensive usage of pesticides in the area is not well managed and monitored. The temperate micro-biotests can be used in tropical systems, but with further research on suitable organisms and standardized methods.

这项研究是在KVRS中进行的；评估水，土壤和沉积物样品的季节性生态毒性；确定在热带系统中使用温带生物测试电池的适用性；使用模糊规则对样品的毒性进行分类，以估计潜在的生态风险。在干旱和雨季期间，在人工林地区收集了143个水，沉积物和土壤样品。门Pseudokirchneriella subcapitata，Aliivibrio鲵和球形节杆菌的生物测定来评估样品的毒性。将结果分类并分类为毒性类别。

在细菌生物测定中，旱季的平均抑制率比雨季样品的平均抑制率高出5％（31％）（p <0.001），这表明由于洪水和增加的地表径流，污染物的浓度较低。少数沉积物样品导致了100％的球形双歧杆菌抑制作用，这意味着生物在暴露于污染物后在生理上没有活性。73％的样品几乎没有毒性潜在危险，25％的严重危险，1％的严重危险升高，这意味着如果该地区农药的广泛使用未得到良好管理和监控，则KVRS生态系统可能处于危险之中。 。温带微生物测试可用于热带系统，但需要进一步研究合适的生物和标准化方法。