The efficiency of the biological removal of carbon and nitrogen from leachates is determined by the activity of microbial populations present in biological reactors. In this work, a complete characterization of bacterial communities revealed by personal genome machine sequencing (PGM) has been carried out from different points of a nitrification–denitrification process operated in an urban landfill. The leachate fed to the treatment is a mixture of young leachate, old leachate, and effluent from an anaerobic digestion process, in a volume ratio of 1:0.9:0.12, respectively. The anoxic and oxic reactors are followed by an ultrafiltration step. Samples are taken from different points of the process. Results reveal the microbial diversity of the samples, which include detection of minority populations that are difficult to explore by other methods. Bacteria belonging to Bacteroidetes and Proteobacteria are dominant in all the samples analyzed. Proteobacteria represents more than 50% of the total population in all cases. Samples taken after the biological treatment show a significant reduction in the relative abundance of Firmicutes, Tenericutes, and Lentisphaerae phyla in comparation with the initial leachate. The relative abundance of the classes is also studied and the most abundant in the samples are β‐Proteobacteria and Flavobacteria.

中文翻译：

---

垃圾渗滤液的硝化-反硝化处理对细菌群落的元基因组学分析

从渗滤液中生物去除碳和氮的效率取决于存在于生物反应器中的微生物种群的活动。在这项工作中，从城市垃圾填埋场的硝化-反硝化过程的不同角度对个人基因组机器测序（PGM）揭示的细菌群落进行了全面表征。送入处理的渗滤液是厌氧消化过程中的年轻渗滤液，旧渗滤液和流出物的混合物，其体积比分别为1：0.9：0.12。缺氧和含氧反应器之后是超滤步骤。从过程的不同点取样。结果揭示了样品的微生物多样性，其中包括检测其他方法难以探索的少数群体。在所有分析的样品中，属于拟杆菌属和变形杆菌属的细菌占主导地位。在所有情况下，变形杆菌均占总人口的50％以上。经过生物处理后采集的样品显示，与最初的沥出液相比，硬毛菌，腱鞘菌和扁豆菌门的相对丰度显着降低。还研究了类别的相对丰度，样品中最丰富的是β-变形杆菌和黄杆菌。与最初的渗滤液相比，阔叶类植物。还研究了类别的相对丰度，样品中最丰富的是β-变形杆菌和黄杆菌。与最初的渗滤液相比，阔叶类植物。还研究了类别的相对丰度，样品中最丰富的是β-变形杆菌和黄杆菌。