The aim of this study is to evaluate the feasibility of sludge digester effluent as feeding solution to enrich anaerobic ammonium oxidation (anammox) bacteria. The performance of the two parallel pilot scale‐upflow packed bed anammox reactors (UPBAn1 and UPBAn2) are examined in terms of the enrichment of anammox bacteria. The control experiment is set up conducting synthetic wastewater as feeding solution in the UPBAn1 reactor whereas, the sludge digester effluent is fed to the nitritation reactor and then the partially nitrated digester effluent to the UPBAn2 reactor. Anammox activities are evaluated by mass balances based on ammonium (NH₄⁺), nitrite (NO₂⁻), and nitrate (NO₃⁻) analysis and NRR. Microbial community of anammox bacteria is analyzed using real‐time polymerase chain reaction (PCR). The results demonstrate that UPBAn 1 and UPBAn2 reactors are successfully enriched on days 64 and 40 with NRRs of 19.54 and 19.43 g N m⁻³ per day, respectively. This study reveals that both synthetic wastewater and digester effluent are suitable for the enrichment of anammox bacteria; however, digester effluent as feeding solution for enrichment of anammox bacteria based on the ease of process control and process stability is more advisable.

中文翻译：

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污泥消化池废水作为厌氧菌富集喂养液的评价

这项研究的目的是评估污泥消化池废液作为进料溶液以富集厌氧铵氧化（anammox）细菌的可行性。根据厌氧菌的富集情况，检查了两个平行的中试规模上流式填充床厌氧菌反应器（UPBAn1和UPBAn2）的性能。建立了控制实验，将合成废水作为进料溶液在UPBAn1反应器中进行处理，而污泥消化池的出水则被送入硝化反应器，然后将部分硝化的消化池的出水入UPBAn2反应器。厌氧氨氧化活性通过基于铵质量平衡（NH评价₄ ⁺），亚硝酸根（NO ₂ ^- ，和硝酸盐（NO）₃ ^-）分析和NRR。使用实时聚合酶链反应（PCR）分析了厌氧细菌的微生物群落。结果表明，UPBAn 1和UPBAn2反应器在第64天和第40天成功富集，其NRR分别为每天19.54和19.43 g N m ^-3。这项研究表明，合成废水和消化池废水都适合富集厌氧氨氧化细菌。然而，基于过程控制的简便性和过程稳定性的考虑，更建议使用消化液作为厌氧菌富集的进料溶液。