A study was conducted in eighteen sheep divided into three groups of six animals each and fed on a diet comprising finger millet (Eleusine coracana) straw and concentrate in 50:50. Animals in the control group (T₀) received diet without tanniferous tree leaves, while in T₁ and T₂ groups, animals received diets with Syzygium cumini (source of HT) and Machilus bombycina (source of CT), respectively. Enteric methane emission from the animals was measured. Hydrolyzable and condensed tannin intake in the T₁ and T₂ groups was 14.08 and 4.29 g/kg dry matter, respectively. Results from the study did not reveal any adverse impact of hydrolyzable and condensed tannin-containing leaves on the dry matter (g/100 kg BW) or digestible dry matter intake. The study confirmed a significant reduction in enteric methane emission (g/d) due to the supplementation. The methanogen numbers in test groups T₁ and T₂ were lower than those in the control; however, the rumen protozoal population (× 105/mL) was adversely affected by Syzygium cumini. Supplementation of selected leaves would help in conserving additional energy for the animal's use and also to curtail the contribution of livestock to GHG emissions. Long-term studies are warranted with selected sources to confirm the persistency of methane reduction.

在十八只绵羊中进行了一项研究，每只绵羊分为三组，每组六只动物，并以50:50的指谷粉（Eleusine coracana）秸秆和浓缩饲料为食。对照组（T ₀）的动物饮食不含丹参，而在T ₁和T ₂组，动物的饮食分别为Syzygium cumini（HT来源）和Machilus bombycina（CT来源）。测量了动物的肠内甲烷排放量。T ₁和T _2中可水解的缩合单宁摄入量每组干物质分别为14.08和4.29 g / kg。研究结果没有显示可水解和压缩的单宁叶对干物质（g / 100 kg BW）或可消化干物质摄入量有任何不利影响。研究证实，由于补充，肠内甲烷排放量（g / d）大大降低。实验组T ₁和T ₂的产甲烷菌数量低于对照组。然而，孜然酵母对瘤胃原生动物种群（×105 / mL）产生了不利影响。补充精选的叶子将有助于节省额外的能量供动物使用，并减少牲畜对温室气体排放的贡献。必须对选定的来源进行长期研究，以确认甲烷还原的持久性。