We study the phylogeography of the series Fissa species in the Iberian Peninsula. This group constitutes a complex formed by three Iberian endemic and threatened taxa with restricted distribution ranges and relatively small population sizes. We amplify and sequence two plastid regions, trnS-trnG and psbA-trnH from 15 sampling locations (n = 45). A total of 15 haplotypes are detected. The median-joining phylogenetic network shows two main haplotype lineages: one western corresponding to the western taxon, D. fissum subsp. sordidum, and the other eastern corresponding to eastern taxa D. bolosii and D. mansanetianum. AMOVA analysis with 94.32% of the total variation and SAMOVA analysis with a Fct value of 0.943 support the presence of these well-defined lineages. We report high values of total gene diversity (h_T = 0.959). Haplotype diversity within populations is low (h_S = 0.311). On the contrary, differentiation among populations (G_ST = 0.675) and Fixation Index (F_ST = 0.989) are high. These values mean that gene flow among populations is limited. Estimation of divergence times shows that the split between D. fissum subsp. sordidum and the two other taxa (D. bolosii and D. mansanetianum) took place during the transition between Pliocene and Pleistocene (approximately 2.67 Ma). Negative Tajima’s D-values test (Tajima’s D = -0.108, P ≥ 0.1) and unimodal mismatch distribution suggest expansion range for D. fissum subsp. sordidum. We advocate to draw up recovery plans for series Fissa species in the Iberian Peninsula, especially for D. fissum subsp. sordidum.

我们研究了伊比利亚半岛系列Fissa物种的系统地理学。该群体由三个伊比利亚地方病和受威胁的生物分类组成，分布范围受限制，人口规模相对较小。我们从15个采样位置（n = 45）扩增了两个质体区域，即trn S- trn G和psb A- trn H并对其进行了测序。总共检测到15个单倍型。中位连接的系统发育网络显示了两个主要的单体型谱系：一个对应于西部分类群D. fissum subsp的西部。sordidum和另一东部对应于东部分类群D. bolosii和D. mansanetianum。具有94.32％总变异的AMOVA分析和Fct值为0.943的SAMOVA分析都支持这些明确定义的谱系。我们报告全基因多样性指数（H的高值_牛逼= 0.959）。种群内的单倍型多样性较低（h _S = 0.311）。相反，人群之间的差异（G _ST = 0.675）和注视指数（F _ST = 0.989）高。这些值意味着种群之间的基因流动是有限的。发散时间的估计表明，D。fissum亚种之间的分裂。sordidum和另外两个分类群（D. bolosii和D. mansanetianum）发生在上新世和更新世之间的过渡期（约2.67 Ma）。负田岛的D值检验（田岛的D = -0.108，P≥0.1）和单峰不匹配分布表明，D。fissum亚种的扩展范围。sordidum。我们主张制定复苏计划中一系列奥栲在伊比利亚半岛的物种，特别是对D. fissum亚种。sordidum。