ABSTRACT Bacterial consortium volatile suspended soil (VSS) (Vatva soil sample) with a capability of azo dye Reactive Orange M2R (ROM2R) decolorization and degradation (shown in our earlier studies using Fourier transform infrared spectroscopy (FTIR) and phytotoxicity studies) was isolated from industrial wastewaters by enrichment culture technique. The present study was carried out to study bacterial population dynamics in consortium Vatva soil sample (VSS) during azo dye ROM2R degradation and to identify the consortium members that were actively involved in the degradation process. To achieve this goal, a real-time Polymerase chain reaction (PCR) assay targeting species-specific region of 16S rDNA of each consortial bacteria was developed to provide quantitative information about the bacterial abundance during azo dye degradation. The real-time PCR assay indicated that Pseudomonas aeruginosa (VSS-6) dominated consortium bacterial community during the active continuous bioremediation process. Attempt has been made to scale up from 100 ml volume to 10 L operation volume with intermittent additions (batch fed loadings) in a Sequence batch reactor (SBR). The development of VSS consortium biomass (MLVSS), changes in COD and biochemical oxygen demand, and the dye degradation were studied under conditions simulating the operations of biological effluent treatment in an attempt to develop a commercially applicable dye effluent treatment process unit.

中文翻译：

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使用实时定量 PCR 参与反应性橙 M2R 染料降解的细菌群落动态，并使用序列分批反应器进行放大研究

摘要 具有偶氮染料活性橙 M2R (ROM2R) 脱色和降解能力的细菌聚生体挥发性悬浮土壤 (VSS)（Vatva 土壤样品）（在我们使用傅里叶变换红外光谱（FTIR）和植物毒性研究的早期研究中显示）是从用富集培养技术处理工业废水。本研究旨在研究偶氮染料 ROM2R 降解过程中联合 Vatva 土壤样品 (VSS) 中的细菌种群动态，并确定积极参与降解过程的联合成员。为了实现这一目标，开发了一种实时聚合酶链反应 (PCR) 检测，其针对每个聚生细菌的 16S rDNA 的物种特异性区域，以提供有关偶氮染料降解过程中细菌丰度的定量信息。实时 PCR 测定表明，铜绿假单胞菌 (VSS-6) 在主动连续生物修复过程中主导了群落细菌群落。已经尝试在序列间歇式反应器 (SBR) 中通过间歇添加（间歇进料加载）将 100 ml 体积扩大到 10 L 操作体积。在模拟生物污水处理操作的条件下研究了 VSS 联合生物质 (MLVSS) 的发展、COD 和生化需氧量的变化以及染料降解，以尝试开发一种商业适用的染料污水处理工艺单元。已经尝试在序列间歇式反应器 (SBR) 中通过间歇添加（间歇进料加载）将 100 ml 体积扩大到 10 L 操作体积。在模拟生物污水处理操作的条件下研究了 VSS 联合生物质 (MLVSS) 的发展、COD 和生化需氧量的变化以及染料降解，以尝试开发一种商业适用的染料污水处理工艺单元。已经尝试在序列间歇式反应器 (SBR) 中通过间歇添加（间歇进料加载）将 100 ml 体积扩大到 10 L 操作体积。在模拟生物污水处理操作的条件下研究了 VSS 联合生物质 (MLVSS) 的发展、COD 和生化需氧量的变化以及染料降解，以尝试开发一种商业适用的染料污水处理工艺单元。