Abstract Biodegradation of phenol has been investigated using a bacterial consortium consisting of two bacterial isolates; one of them used for the first time in phenol biodegradation. This consortium was isolated from activated sludge and identified as Providencia stuartii PL4 and Pseudomonas aeruginosa PDM (accession numbers KY848366 and MF445102, respectively). The degradation of phenol by this consortium was optimal at pH 7 with using 1500 mg l−1 ammonium chloride as a nitrogen source. Interestingly, after optimizing the biodegradation conditions, this consortium was able to degrade phenol completely up to 1500 mg l−1 within 58 h. The immobilization of this consortium on various supporting materials indicated that polyvinyl alcohol (PVA)-alginate beads and polyurethane foam (PUF) were more suitable for biodegradation process. The freely suspended cells could degrade only 6% (150 mg l−1) of 2500 mg l−1 phenol, whereas, the immobilized PVA-alginate beads and the immobilized PUF degraded this concentration completely within 120 h of incubation with degradation rates (q) 0.4839 and 0.5368 (1/h) respectively. Thus, the immobilized consortium of P. stuartii PL4 and P. aeruginosa PDM can be considered very promising in the treatment of effluents containing phenol.

中文翻译：

---

从活性污泥中分离的普罗维登西亚 PL4 和铜绿假单胞菌 PDM 的游离和固定混合培养增强苯酚降解

摘要 使用由两种细菌分离物组成的细菌聚生体研究了苯酚的生物降解；其中之一首次用于苯酚生物降解。该聚生体从活性污泥中分离出来并被鉴定为普罗维登西亚斯图亚特 PL4 和铜绿假单胞菌 PDM（登录号分别为 KY848366 和 MF445102）。使用 1500 mg l-1 氯化铵作为氮源，该联合体对苯酚的降解在 pH 值为 7 时最佳。有趣的是，在优化生物降解条件后，该联盟能够在 58 小时内完全降解高达 1500 mg l-1 的苯酚。该财团在各种支持材料上的固定表明，聚乙烯醇（PVA）-海藻酸盐珠和聚氨酯泡沫（PUF）更适合生物降解过程。自由悬浮的细胞只能降解 6% (150 mg l-1) 的 2500 mg l-1 苯酚，而固定化 PVA-藻酸盐珠和固定化 PUF 在孵育 120 小时内以降解速率（q ) 0.4839 和 0.5368 (1/h)。因此，P. stuartii PL4 和 P. aeruginosa PDM 的固定化聚生体在处理含苯酚的废水中被认为是非常有前途的。