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Synergistic effect of bacterial consortium on the biodegradation of nitroglycerin in aqueous medium through laboratory-scale bioreactor process
Bioremediation Journal ( IF 1.9 ) Pub Date : 2019-07-03 , DOI: 10.1080/10889868.2019.1638340
G. Vanitha 1 , S. Rajakumar 2 , P. M. Ayyasamy 1
Affiliation  

Abstract Nitroglycerin (NG) degrading bacterial strains were isolated from the soil samples obtained from contaminated regions of Salem and Sivakasi, Tamil Nadu, India. The isolated strains were tested for their ability to degrade of nitroglycerin in mineral salts medium at optimum temperature. Among 75 strains isolated, three strains were revealed more potent on the degradation of nitroglycerin. They were selected based on their efficiency in the breakdown of nitrogen molecules, the tolerant ability of glycerin and antagonistic assay. The selected strains were identified as Bacillus sp. and Pseudomonas sp. The mineral salts medium amended with NG was used to find out the optimum carbon source, pH and temperature for the degradation of NG. The rate NG degradation was found to be higher by using 1% glucose, pH 7.0 and 30 °C as the appropriate factors. Treated water from the bioreactor study was characterized using UV-Vis spectrophotometer, FTIR, HPLC and GC/MS analysis, which showed a potential degradation of NG by the bacterial consortium. Phyto-toxicity tests revealed that significant growth of green gram in the pots irrigated with treated water.

中文翻译:

通过实验室规模的生物反应器过程,细菌聚生体对水介质中硝酸甘油的生物降解的协同作用

摘要 从印度泰米尔纳德邦塞勒姆和西瓦卡西污染地区获得的土壤样品中分离出硝酸甘油 (NG) 降解菌株。测试分离的菌株在最佳温度下在矿物盐培养基中降解硝酸甘油的能力。在分离的 75 株菌株中,有 3 株对硝酸甘油的降解作用更强。选择它们是基于它们在氮分子分解中的效率、甘油的耐受能力和拮抗试验。所选菌株被鉴定为芽孢杆菌属。和假单胞菌。用经 NG 改性的矿物盐培养基寻找降解 NG 的最佳碳源、pH 值和温度。通过使用 1% 葡萄糖,pH 7,发现 NG 降解率更高。0 和 30 °C 作为适当的因素。使用紫外-可见分光光度计、FTIR、HPLC 和 GC/MS 分析对来自生物反应器研究的处理水进行表征,结果表明细菌聚生体可能会降解 NG。植物毒性试验表明,在用处理过的水灌溉的花盆中,绿豆显着生长。
更新日期:2019-07-03
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