Abstract Locally, the petroleum, aluminum and steel milling industries have expressed considerable interest in the utilization of bioremediation as a means for reducing their lubricant waste. Since hydrocarbons accumulate within microalgae, the aim of this study was to determine the efficiency of Scenedesmus vacuolatus vs a microalgal consortium (Chlamydomonas pitschmannii, Trebouxia australis and Pectinodesmus pectinatus) in the biodegradation lubricant waste products; from which these indigenous microalgae were originally isolated. To determine the biodegradation capability of these microalgae, dehydrogenase activity tests were carried out and indicated by % TPF values. Samples that tested positive for dehydrogenase activity, were analyzed using gas chromatography/mass spectrometry, to track the biotransformation of hydrocarbons intracellularly and extracellularly. Results were analyzed statistically with SPSS, p < 0.05. Dehydrogenase activity for both the coolant rolling oil (0.058 ± 0.001 mg/mL), and the spent rolling emulsion (0.047 ± 0.002) was higher for the microbial consortium compared to S. vacuolatus, 0.046 ± 0.001 mg/mL. Weekly, gas chromatography/mass spectrometry biotransformations, indicated no hydrocarbons present after five weeks, confirming 100% biodegradation. Irrespective of the use of either a single isolate or a microalgal consortium; complete biodegradation after just five weeks, makes the use of microalgae for bioremediation a viable strategy.

中文翻译：

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空泡栅藻与微藻聚生体对润滑剂废物的生物降解的比较

摘要 在当地，石油、铝和钢铁加工行业对利用生物修复作为减少润滑剂浪费的一种手段表示了极大的兴趣。由于碳氢化合物在微藻内积累，本研究的目的是确定 Scenedesmus vacuolatus 与微藻聚生体（Chlamydomonas pitschmannii、Trebouxia australis 和 Pectinodesmus pectinatus）在生物降解润滑剂废物产品中的效率；这些本土微藻最初是从中分离出来的。为了确定这些微藻的生物降解能力，进行了脱氢酶活性测试并用 % TPF 值表示。脱氢酶活性检测呈阳性的样品使用气相色谱/质谱分析，在细胞内和细胞外跟踪碳氢化合物的生物转化。结果用SPSS进行统计学分析，p < 0.05。微生物聚生体的冷却轧制油 (0.058 ± 0.001 mg/mL) 和废轧制乳液 (0.047 ± 0.002) 的脱氢酶活性高于 S. vacuolatus 0.046 ± 0.001 mg/mL。每周，气相色谱/质谱生物转化表明五周后没有碳氢化合物存在，证实 100% 生物降解。无论是使用单一分离物还是微藻聚生体；仅五周后即可完全生物降解，这使得使用微藻进行生物修复成为一种可行的策略。与 S. vacuolatus 相比，微生物聚生体的废滚动乳液 (0.047 ± 0.002) 更高，为 0.046 ± 0.001 mg/mL。每周，气相色谱/质谱生物转化表明五周后没有碳氢化合物存在，证实 100% 生物降解。无论是使用单一分离物还是微藻聚生体；仅五周后即可完全生物降解，这使得使用微藻进行生物修复成为一种可行的策略。与 S. vacuolatus 相比，微生物聚生体的废滚动乳液 (0.047 ± 0.002) 更高，为 0.046 ± 0.001 mg/mL。每周，气相色谱/质谱生物转化表明五周后没有碳氢化合物存在，证实 100% 生物降解。无论是使用单一分离物还是微藻聚生体；仅五周后即可完全生物降解，这使得使用微藻进行生物修复成为一种可行的策略。