Abstract The oil industry is responsible for the contamination of vast areas of soil and sea. Although crude oil spills in the environment have a huge visual appeal, it is the daily contamination, by diary activities involving oil exploration, transportation and refining that causes more damage to the environment. Bioremediation technologies, especially the use of biosurfactant molecules, have been shown to be more efficient than other traditional technologies. The objective of this research was to analyze the characteristics of the biosurfactants molecules produced by Pseudomonas putida AM-b1 strain and to evaluate their application in the remediation of environments contaminated by crude oil. The AM-b1 strain isolated from the marine environment, in an area contaminated by hydrocarbons, was evaluated in relation to biosurfactant production with different oils as carbon sources, as well as its emulsification action on crude oil by microbial enhanced oil recovery (MEOR) experiment. The laboratory results showed that the strain’s biosurfactant maintains its emulsification action even under variations in conditions, such as pH, high concentrations of NaCl, previous exposure to ultraviolet radiation and autoclaving processes. In addition, the MEOR experiments showed that the P. putida strain’s biosurfactant has the potential to be used in oil recovery.

中文翻译：

---

油降解假单胞菌 AM-b1 菌株生产的生物表面活性剂具有微生物提高采收率的潜力

摘要 石油工业对大面积的土壤和海洋造成污染。环境中的原油泄漏虽然具有巨大的视觉吸引力，但对环境造成更大破坏的是日常污染，包括石油勘探、运输和炼油等日常活动。生物修复技术，尤其是生物表面活性剂分子的使用，已被证明比其他传统技术更有效。本研究的目的是分析恶臭假单胞菌 AM-b1 菌株产生的生物表面活性剂分子的特性，并评估其在修复原油污染环境中的应用。从海洋环境中分离出的 AM-b1 菌株，位于被碳氢化合物污染的区域，通过微生物提高采收率 (MEOR) 实验评估了以不同油为碳源的生物表面活性剂生产及其对原油的乳化作用。实验室结果表明，该菌株的生物表面活性剂即使在条件变化（例如 pH 值、高浓度 NaCl、先前暴露于紫外线辐射和高压灭菌过程）下也能保持其乳化作用。此外，MEOR 实验表明恶臭假单胞菌菌株的生物表面活性剂具有用于采油的潜力。高浓度 NaCl、之前暴露于紫外线辐射和高压灭菌过程。此外，MEOR 实验表明恶臭假单胞菌菌株的生物表面活性剂具有用于采油的潜力。高浓度 NaCl、之前暴露于紫外线辐射和高压灭菌过程。此外，MEOR 实验表明恶臭假单胞菌菌株的生物表面活性剂具有用于采油的潜力。