Abstract A marine bacterial isolate, Bacillus vietnamensis sp. MSB17 from the sediments collected from the continental slope of the eastern Arabian Sea exhibited good Malachite Green (MG) decolorization ability in mineral medium supplemented with glucose and yeast extract under static conditions. The complete decolorization of dye (50 mg/L) was attained within 4 h of incubation. The activities of enzymes such as tyrosinase (0.62 IU/ml), laccase (12.02 U/ml), and manganese peroxidase (6.23 U/ml) were observed during dye degradation. UV-VIS, FT-IR, and GC-MS analysis of dye samples extracted with ethyl acetate confirmed the biodegradation of MG into methanone, [4-(dimethylamino) phenyl] phenyl- and 2, 6-bis (1, 1-dimethylethyl) phenol. The metabolites obtained from B. vietnamensis sp. MSB17 mediated MG degradation did not exhibit any microbial toxicity and phytotoxicity. The present study indicated the efficiency of Bacillus vietnamensis sp. MSB17 to effectively degrade MG, revealing its potential to be further utilized for bioremediation purposes.

中文翻译：

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新分离的越南芽孢杆菌对孔雀石绿的生物降解。来自东阿拉伯海大陆坡的 MSB17：酶分析、降解途径和毒性研究

摘要 一种海洋细菌分离物，越南芽孢杆菌。来自阿拉伯海东部大陆坡的沉积物中的 MSB17 在静态条件下在添加葡萄糖和酵母提取物的矿物培养基中表现出良好的孔雀石绿 (MG) 脱色能力。在孵育 4 小时内实现染料的完全脱色 (50 毫克/升)。在染料降解过程中观察到酪氨酸酶 (0.62 IU/ml)、漆酶 (12.02 U/ml) 和锰过氧化物酶 (6.23 U/ml) 等酶的活性。对用乙酸乙酯提取的染料样品进行 UV-VIS、FT-IR 和 GC-MS 分析证实 MG 生物降解为甲酮、[4-(二甲氨基)苯基]苯基-和 2, 6-双 (1, 1-二甲基乙基) ) 苯酚。从 B. vietnamensis sp. 获得的代谢物。MSB17 介导的 MG 降解没有表现出任何微生物毒性和植物毒性。本研究表明越南芽孢杆菌的效率。MSB17 可有效降解 MG，揭示其进一步用于生物修复目的的潜力。