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Strategies for Fermentable Sugar Production by Using Pressurized Acid Hydrolysis for Rice Husks
Rice Science ( IF 5.6 ) Pub Date : 2019-09-09 , DOI: 10.1016/j.rsci.2019.08.006
Giovanni B. Pedroso , Michael R. Philippsen , Loisleini F. Saldanha , Raiara B. Araujo , Ayrton F. Martins

This study investigated the use of leftover biomass (rice husks) as the raw material for the biotechnological production of platform chemicals and biopolymers. Following the biorefinery concept, different acid hydrolysates were studied and resulted into a wide range of treatment strategies. Chemometrics were applied throughout the procedures in multivariate experimental conditions. By using the best hydrolytic conditions of 6.0% H3PO4, 135 °C (45 MPa) and reaction time of 62 min, 21.0 g/L sugar hydrolysates were produced; by using the best hydrolytic condition of 4.5% HNO3, 135 °C/35 min, 16.1 g/L sugar hydrolysates were produced; and with the hydrolysates use of 1.5% H2SO4 and 1.5% HCl, 135 °C/62 min, 18.2 and 17.8 g/L sugar hydrolysates were produced, respectively. The highest productivity, in terms of fermentable sugars, reached 68% of integral cellulose/hemicellulose fraction and surpassed those found in the literature, with regard to the processing of rice husks, by considering just one step process. Sulfuric hydrolysate, detoxified with active carbon, was used to prove this proposal viability, resulting in a fermentation substrate for A. terreus (ATCC10020) and R. radiobacter (LMG196) strains (natural producers of bioproducts), which certified the feasibility of the proposal. The production of fermentable sugars from leftover biomass should encourage a search for new bioconversion routes, which can result in economic and environmental benefits and a spread of knowledge.



中文翻译:

稻壳加压酸水解生产可发酵糖的策略

这项研究调查了使用剩余的生物质(稻壳)作为生物技术生产平台化学品和生物聚合物的原料。遵循生物精炼概念,对不同的酸水解产物进行了研究,并得出了广泛的处理策略。化学计量学在整个实验过程中以多​​元实验条件应用。通过使用6.0%H 3 PO 4的最佳水解条件,135°C(45 MPa)和62分钟的反应时间,可制得21.0 g / L的糖水解物。通过使用最佳水解条件为4.5%HNO 3,在135°C / 35 min时,生产了16.1 g / L的糖水解物。并使用1.5%H 2 SO 4的水解产物分别在135°C / 62分钟,18.2 g / L和17.8 g / L的糖水解产物中生成1.5%HCl。就可发酵糖而言,仅考虑一步法,就稻壳的加工而言,最高生产率就达到了纤维素/半纤维素总含量的68%,并超过了文献中发现的生产率。用活性炭解毒的硫酸水解液用于证明该建议的可行性,从而形成A的发酵底物。土曲霉(ATCC10020)和- [R 放射性细菌(LMG196)菌株(生物产品的天然生产者),证明了该建议的可行性。由剩余生物质生产可发酵糖应鼓励寻找新的生物转化途径,这可以带来经济和环境效益以及知识传播。

更新日期:2019-09-09
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