The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.

将野生染色体片段渗入流行的水稻品种是开发用于干旱胁迫条件的品种的潜在途径之一。选择了16种基因型，包括9种in稻，2种热带粳稻和5种对干旱胁迫具有不同耐受性/敏感性的染色体片段替代品系（CSSLs）进行多样性研究。利用63个微卫星标记来评估遗传多样性。总共扩增了95个等位基因，其中60个是多态性。六个独特的等位基因，通过微卫星位点RM276，RM472，RM488，RM537，RM541和RM28089放大，在六个基因型鉴定即FR13A，Brahamanakhi，RUF44，Swarna- SUB1，婆罗门那喜和萨蒂雅哈玛。在CSSL中发现了最高的遗传相似性。多态信息含量（PIC）值从0到1.00不等，平均每个位点为0.66。发现28个微卫星具有多态性，可用于标记辅助选择程序。所有这16个基因型都以0.64的遗传相似性分为两个主要的簇。在第一类中，被鉴定为不同基因型的五个CSSL具有负责抵抗干旱的野生祖先片段，因此它们可以用作潜在的供体。印度流行的Swarna- sub1和IR64- sub1品种可以在将来的育种计划中用作轮回亲本，以开发用于淹没和干旱等非生物胁迫的品种。