Activated carbon (AC) is a known adsorbent for organic compounds including root exudates but could have an influence on crop yield and greenhouse gas (GHG) emissions. A 2-year pot experiment was conducted to assess the effect of AC on GHG emissions, seed yield, soil chemical properties and isoflavone content of soybean genotypes with varying nodulation capacities under sandy soil conditions. We also evaluated the nodulation and seed protein content after harvest. The soybean genotypes were TnVRSN4, Tachinagaha and TnVRNN4 with high, normal and low nodulation capacities respectively. AC was applied at rates equivalent to 0, 2.4, 4.8, and 9.6 t ha⁻¹ in combination with inorganic fertilizers. The results showed that AC tended to reduce soil N₂O emissions in the high nodulating genotype due to the significant reduction in nodulation but did not significantly affect CO₂ and CH₄ emissions. Highest CO₂ emissions and seed yield were observed in the high nodulating genotype and lowest in the low nodulating genotype. AC did not significantly affect seed yield of the high nodulating genotype but significantly reduced seed yield of the low and normal nodulation genotypes in 2017 and 2018 respectively. Although AC generally increased soil total N, total C and C/N ratio, its effect on soil pH, available P and exchangeable cations significantly varied with the soybean genotype. AC did not significantly affect root isoflavone, seed protein and total isoflavone content but significantly reduced the concentration of daidzein and daidzin which were exuded from soybean roots in soil. The high nodulating genotype can perform better in sandy soils with a low nutrient status but further studies using pyrogenic carbonaceous soil amendments are needed to evaluate and compare the three genotypes in terms of productivity and GHG emissions under field conditions, as well as assessing the feasible chemical N fertilizer application rates for the low nodulating genotype.

活性炭（AC）是有机化合物（包括根系分泌物）的已知吸附剂，但可能会对作物产量和温室气体（GHG）排放产生影响。进行了为期两年的盆栽试验，以评估在沙质土壤条件下，不同根瘤能力下，AC对大豆基因型温室气体排放，种子产量，土壤化学性质和异黄酮含量的影响。我们还评估了收获后的结瘤和种子蛋白质含量。大豆的基因型分别为高结瘤能力，正常结瘤能力和低结瘤能力的TnVRSN4，Tachinagaha和TnVRNN4。与无机肥料组合使用时，AC的添加量相当于0、2.4、4.8和9.6 t ha ^-1。结果表明，AC能减少土壤N ₂含量高根瘤基因型中的O排放是由于根瘤形成显着减少，但并未显着影响CO ₂和CH ₄排放。最高CO ₂在高根瘤基因型中观察到排放量和种子产量，而在低根瘤基因型中观察到最低的产量。AC不会显着影响高结瘤基因型的种子产量，但分别在2017年和2018年显着降低了低结瘤基因型和正常结瘤基因型的种子产量。尽管AC通常会增加土壤总氮，总C和C / N比，但其对土壤pH值，有效磷和可交换阳离子的影响随大豆基因型的不同而显着不同。AC对大豆根的异黄酮，种子蛋白和总异黄酮含量没有明显影响，但可以显着降低大豆根在土壤中渗出的大豆苷和大豆苷的浓度。