This study aimed to characterize endophytic bacteria associated with root-nodules of a wild legume (Genista cinerea: Fabaceae) growing in arid soils of Algeria. A total of ten non-symbiotic endophytic bacterial strains were isolated and identified using a combination of conventional and molecular approaches based on 16S rRNA gene sequencing. Moreover, growth variations of the isolates under different environmental conditions were examined using advanced statistical modeling techniques (Markov Chain Monte Carlo sampler for multivariate generalized linear mixed models-MCMCglmm). The results revealed the existence of a large physiological diversity among the endophyte isolates, which formed two distinct groups. Group 1, included fast-growing and salt-tolerant isolates and Group 2 covered acid-sensitive isolates. The bacterial isolates showed capabilities to assimilate different carbonaceous and nitrogenous substances, with consistent and large tolerances to pH [4–10], temperature [4–55 °C], and salinity [NaCl = 2–10%]. In addition, The MCMCglmm indicated that scored growth rate increased with pH and decreases at high level of salinity. The endophytic strains were classified as fast-growing bacteria belonged to the β and γ-Proteobacteria including Achromobacter, Klebsiella, Luteibacter, Pantoea and Pseudomonas. The present findings may support the idea that these isolates are passenger bacteria able to colonize nodules of Genista cinerea. Furthermore, the salt and temperature tolerant patterns found among the isolates reflect the environmental stresses pressure and the importance of using efficient indigenous endophytic strains for successful plant inoculation in arid agriculture.

这项研究旨在表征与野生豆科植物（Genista cinerea）根瘤相关的内生细菌：豆科）在阿尔及利亚的干旱土壤中生长。使用常规方法和基于16S rRNA基因测序的分子方法相结合，共分离和鉴定了十种非共生内生细菌菌株。此外，使用先进的统计建模技术（马尔可夫链蒙特卡洛采样器，用于多元广义线性混合模型-MCMCglmm），检查了分离物在不同环境条件下的生长变化。结果表明，内生菌分离物之间存在较大的生理多样性，形成了两个不同的群体。第1组包括生长迅速且耐盐的分离株，第2组覆盖了对酸敏感的分离株。细菌分离物具有吸收不同碳质和氮质物质的能力，对pH值[4-10]，温度[4-55°C]和盐度[NaCl = 2-10％]具有一致且较大的公差。此外，MCMCglmm表示，随着pH的升高，刻划的生长速率增加，而在高盐度下，刻划的生长速率降低。内生菌株被分类为属于β和γ-的快速生长细菌变形菌，包括无色杆菌，克雷白杆菌，Luteibacter，成团泛菌和假单胞菌。目前的发现可能支持这些分离物是能够侵染灰Gen（Genista cinerea）结节的细菌。此外，在分离物中发现的耐盐和耐温模式反映了环境胁迫压力以及在干旱农业中成功使用有效的本地内生菌株成功接种植物的重要性。