Abstract Mysids are experimental models and are among the most important food items for animals in aquaria and that support fisheries, and even for humans, but information on their performance in controlled culture systems is still limited. We reared the marine mysid Neomysis awatschensis in a controlled laboratory system, and measured its growth, 20–hydroxyecdysone (20E) levels, molting, and survival in response to environmental fluctuations in temperature, pH, and salinity, and inferred their potential associations based on annual field sampling. The 20E levels were significantly elevated during the postnauplioid stages, and even higher levels of 20E were maintained in the adult stages than in the nauplioid stages. Values of growth parameters (i.e. total length and the lengths of the antennal scale, expod, endopod, and telson) and 20E levels were higher during a 40-day period at 25 °C than at other temperatures, with shorter intermolt intervals, although morality was also increased. Among the surviving mysids, the number of newly hatched juveniles produced was higher for females exposed to 20 °C than that in other groups. Relatively higher growth and survival rates were measured at salinities over 25 practical salinity, while lower salinities under 15 practical salinity significantly reduced growth and survival. The number of newly hatched juveniles was lower at salinities under 15 practical salinity compared to those over 20 practical salinity. Overall, low temperature and salinity reduced mysid reproduction and the maintenance of the second generation. In the case of pH variation (pH of 7.0–8.0), there were no significant effects on growth and the number of newly hatched juveniles, although the survival rate was slightly lower and the 20E level fluctuated at a pH of 7.0. We believe that these associations between growth and environmental conditions can provide crucial information for optimizing mass mysid culture for experimental and ecotoxicological usage in the laboratory.

中文翻译：

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环境波动对海洋 Mysid Neomysis awatschensis 的影响分析及其作为实验模型动物的发展

摘要 Mysids 是实验模型，是水族馆中动物最重要的食物之一，支持渔业，甚至是人类，但关于它们在受控养殖系统中的表现的信息仍然有限。我们在受控实验室系统中饲养了海洋贻贝 Neomysis awatschensis，并测量了它的生长、20-羟基蜕皮激素 (20E) 水平、蜕皮和存活率，以响应温度、pH 和盐度的环境波动，并根据以下数据推断它们的潜在关联每年实地取样。20E 水平在后无节幼体阶段显着升高，并且在成体阶段维持的 20E 水平甚至高于无节幼体阶段。生长参数的值（即总长度和触角鳞片、外足、内足、和 telson) 和 20E 水平在 25 °C 的 40 天期间比在其他温度下更高，换羽间隔更短，尽管道德也有所提高。在幸存的糠虾中，暴露于 20°C 的雌性产生的新孵化的幼鱼数量高于其他组。在超过 25 实际盐度的盐度下测得相对较高的生长和存活率，而低于 15 实际盐度的较低盐度显着降低了生长和存活率。与实际盐度超过 20 的盐度相比，在 15 实际盐度以下的盐度下，新孵化的幼鱼数量较低。总体而言，低温和盐度降低了糠虾繁殖和第二代的维持。在 pH 变化（pH 7.0-8.0）的情况下，对生长和新孵化的幼鱼数量没有显着影响，尽管成活率略低，20E水平在7.0的pH值波动。我们相信，生长和环境条件之间的这些关联可以为优化用于实验室实验和生态毒理学用途的大规模糠虾培养提供关键信息。