The aim of this work was to investigate the effect of temperature (40–60 °C), pressure (10–20 MPa) and co-solvent concentration (0–2 wt.%) on extraction yield from L. rivularis leaves using ethanol-modified supercritical carbon dioxide. Extraction assays were carried out in a Process Development Unit using a Box-Behnken design. Extraction yield ranged from 14.7 to 53.1 g/kg dry substrate, with the highest value being achieved at 60 °C, 20 MPa and 2 wt.%. The pressure and the co-solvent concentration had a significant effect on extract recovery. To complement the analysis, recognized bioactive compounds, such as the flavonoids quercetin, kaempferol and resveratrol and the terpenes α-thujone, β-caryophyllene and caryophyllene oxide were quantified in the selected extract. Antioxidant activity and the inhibition of key enzymes linked to diabetes mellitus (α-amylase and α-glucosidase) were exhibited by the extract. These results demonstrate the potential benefit of L. rivularis extract for human health.

这项工作的目的是研究温度（40–60°C），压力（10–20 MPa）和助溶剂浓度（0–2 wt。％）对小叶柳桉提取率的影响。叶使用乙醇改性的超临界二氧化碳。提取测定是在过程开发单元中使用Box-Behnken设计进行的。干燥基质的提取产率为14.7至53.1 g / kg，在60°C，20 MPa和2 wt。％的条件下可获得最高的提取率。压力和助溶剂浓度对提取物的回收率有显着影响。为了补充分析结果，在选定的提取物中对公认的生物活性化合物进行了定量，如黄酮类槲皮素，山res酚和白藜芦醇以及萜烯α-丁酮，β-石竹烯和石竹烯氧化物。提取物表现出抗氧化活性和与糖尿病相关的关键酶（α-淀粉酶和α-葡萄糖苷酶）的抑制作用。这些结果证明了L. rivularis的潜在益处 提取物对人体健康。