Fenugreek (Trigonella-foenum graecum) is a tropical plant with medicinal properties which is traditionally known since the ancient time. In this research, various microwave-assisted extraction (MAE) related parameters such as extraction time (2–12 min), ethanol concentration (20–100%), microwave power (300–700 W), ratio of feed-to-solvent (1:8-1:16 g/mL) and extraction temperature (40–80 °C) were evaluated using experimental method of one-factor-at-a-time (OFAT). The screening of extraction parameters was then evaluated using two-level factorial design under Design-Expert to find out the significant parameters in terms of obtaining high extraction yield, total saponin, phenolic and flavonoid contents in fenugreek seed. The results of OFAT revealed that the maximum yields of recovery were achieved at microwave parameters of 3 min irradiation time, microwave power 600 W, 60% solvent concentration, 1:10 g/mL of feed-to-solvent ratio and 70 °C temperature. The screening process using two level factorial design (TLFD) also indicated that solvent concentration, time of irradiation, microwave power and ratio of feed-to-solvent were the significant parameters affecting the recovery yields of total saponin, phenolic and flavonoid contents where (p < 0.05). While, the temperature of microwave was not counted significant (P > 0.05). The extract was also evaluated for its chemical composition using GC–MS analysis.

胡芦巴（Trigonella-foenum graecum）是一种具有药用特性的热带植物，自古以来就广为人知。在这项研究中，各种微波辅助萃取（MAE）相关参数，例如萃取时间（2–12分钟），乙醇浓度（20–100％），微波功率（300–700 W），进料与溶剂的比例（1：8-1：16 g / mL）和提取温度（40–80°C）使用一次一因素试验（OFAT）的方法进行评估。然后在Design-Expert下使用两级因子设计对提取参数的筛选进行评估，以找出在胡芦巴种子中获得高提取得率，总皂苷，酚和类黄酮含量方面的重要参数。OFAT的结果表明，在微波参数为3分钟的照射时间，微波功率为600 W，60％的溶剂浓度，1：10 g / mL的进料比和70°C温度。使用二级因子设计（TLFD）进行的筛选过程还表明，溶剂浓度，辐照时间，微波功率和进料比是影响总皂苷，酚和类黄酮含量回收率的重要参数，其中（p <0.05）。同时，微波温度未计算出显着性（P> 0.05）。还使用GC-MS分析评估了提取物的化学成分。微波功率和进料比是影响总皂苷，酚类和类黄酮含量回收率的重要参数，其中（p <0.05）。同时，微波温度未计算出显着性（P> 0.05）。还使用GC-MS分析评估了提取物的化学成分。微波功率和进料比是影响总皂苷，酚类和类黄酮含量回收率的重要参数，其中（p <0.05）。同时，微波温度未计算出显着性（P> 0.05）。还使用GC-MS分析评估了提取物的化学成分。