Salicylic acid (SA) is an important signaling substance that plays an important role in plant growth, development and disease resistance. In order to further understand the role of the SA pathway in potato disease resistance and identify SA signaling key genes, gene expression profiling of the late blight resistance genotype SD20 was performed under exogenous SA application. A total of 28 572 unigenes were assembled, of which 4 564 were differentially expressed. Analysis of differentially expressed genes (DEGs) showed that multiple signaling pathways such as SA, jasmonic acid, ethylene, abscisic acid, auxin, and brassinolide were involved in response to exogenous SA. Many plant defense signal-related genes involved in protein serine/threonine kinase activity and plant-pathogen interaction, were significantly enriched. These were consistent with the interaction results of SD20 and Phytophthora infestans in our previous study, indicating that exogenous SA stimulated the resistance response and initiated a similar defense pathway compared to pathogen infection in SD20, which confirmed crosstalk of the SA signaling pathway with a pathogen-induced disease resistance signal pathway in plant. Moreover, transcriptome analysis revealed that ROS1 was positively regulated by SA in potato for the first time. SA-induced gene expression profiling provides insight into SA signaling and its mechanisms in disease defense systems.

水杨酸（SA）是重要的信号传导物质，在植物生长，发育和抗病性中起着重要作用。为了进一步了解SA途径在马铃薯抗病性中的作用并鉴定SA信号转导关键基因，在外源SA应用下对晚疫病抗性基因型SD20进行了基因表达谱分析。总共组装了28572个单基因，其中有4564个差异表达。对差异表达基因（DEG）的分析表明，多种信号途径如SA，茉莉酸，乙烯，脱落酸，生长素和油菜素内酯都参与了对外源SA的反应。与蛋白质丝氨酸/苏氨酸激酶活性和植物-病原体相互作用有关的许多植物防御信号相关基因均被显着富集。在我们以前的研究中，疫霉菌感染表明，与SD20中的病原体感染相比，外源性SA刺激了抗性反应并启动了类似的防御途径，这证实了SA信号通路与病原体诱导的疾病抗性信号通路的串扰。此外，转录组分析首次表明，ROS1受到马铃薯中SA的正调控。SA诱导的基因表达谱分析可深入了解SA信号及其在疾病防御系统中的机制。